L-Leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase
(1983) In American Journal of Physiology - Endocrinology and Metabolism 245(4). p.338-346- Abstract
Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthetised. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the K(m) for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter... (More)
Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthetised. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the K(m) for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter L-glutamine, 1 mmol/liter L-leucine OMe induced a 50- to 75-fold increase in insulin release. A similar stimulatory effect was also observed in column-perifused RIN 5F cells, a cloned rat islet tumor cell line. A twofold increase in islet glutamate dehydrogenase activity was induced by 5 mmol/liter L-leucine OMe, a larger effect than that of L-leucine (P < 0.02), whereas L-arginine OMe had a small inhibitory effect. We conclude that L-leucine OMe is a potent stimulus of insulin secretion and that its effect on the β-cells may be exerted by activating islet glutamate dehydrogenase.
(Less)
- author
- Knudsen, P.
; Kofod, H.
; Lernmark, A.
LU
and Hedeskov, C. J.
- publishing date
- 1983-01-01
- type
- Contribution to journal
- publication status
- published
- in
- American Journal of Physiology - Endocrinology and Metabolism
- volume
- 245
- issue
- 4
- pages
- 338 - 346
- publisher
- American Physiological Society
- external identifiers
-
- pmid:6194691
- scopus:0020840915
- ISSN
- 0193-1849
- language
- English
- LU publication?
- no
- id
- a20d4ef6-35df-48f8-a134-86ac15e88438
- date added to LUP
- 2019-09-16 12:44:45
- date last changed
- 2024-03-13 08:12:36
@article{a20d4ef6-35df-48f8-a134-86ac15e88438, abstract = {{<p>Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthetised. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the K(m) for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter L-glutamine, 1 mmol/liter L-leucine OMe induced a 50- to 75-fold increase in insulin release. A similar stimulatory effect was also observed in column-perifused RIN 5F cells, a cloned rat islet tumor cell line. A twofold increase in islet glutamate dehydrogenase activity was induced by 5 mmol/liter L-leucine OMe, a larger effect than that of L-leucine (P < 0.02), whereas L-arginine OMe had a small inhibitory effect. We conclude that L-leucine OMe is a potent stimulus of insulin secretion and that its effect on the β-cells may be exerted by activating islet glutamate dehydrogenase.</p>}}, author = {{Knudsen, P. and Kofod, H. and Lernmark, A. and Hedeskov, C. J.}}, issn = {{0193-1849}}, language = {{eng}}, month = {{01}}, number = {{4}}, pages = {{338--346}}, publisher = {{American Physiological Society}}, series = {{American Journal of Physiology - Endocrinology and Metabolism}}, title = {{L-Leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase}}, volume = {{245}}, year = {{1983}}, }