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A DNA prism : Physical principles for optimizing a microfabricated DNA separation device

Huang, Lotien Richard ; Tegenfeldt, Jonas O. LU orcid ; Sturm, James C. ; Austin, Robert H. and Cox, Edward C. (2002) 2002 IEEE International Devices Meeting (IEDM) p.211-214
Abstract

Recently, we reported a microfabricated "DNA prism" device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the "biphasic" separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.

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author
; ; ; and
publishing date
type
Contribution to conference
publication status
published
pages
4 pages
conference name
2002 IEEE International Devices Meeting (IEDM)
conference location
San Francisco, CA, United States
conference dates
2002-12-08 - 2002-12-11
external identifiers
  • scopus:0036927877
language
English
LU publication?
no
id
a3b6a336-30a6-49fc-9a5d-123b3550464f
date added to LUP
2018-10-20 10:39:16
date last changed
2022-01-31 06:19:36
@misc{a3b6a336-30a6-49fc-9a5d-123b3550464f,
  abstract     = {{<p>Recently, we reported a microfabricated "DNA prism" device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the "biphasic" separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.</p>}},
  author       = {{Huang, Lotien Richard and Tegenfeldt, Jonas O. and Sturm, James C. and Austin, Robert H. and Cox, Edward C.}},
  language     = {{eng}},
  month        = {{12}},
  pages        = {{211--214}},
  title        = {{A DNA prism : Physical principles for optimizing a microfabricated DNA separation device}},
  year         = {{2002}},
}