Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Is acetylcholine an autocrine/paracrine growth factor via the nicotinic α7-receptor subtype in the human colon cancer cell line HT-29?

Pettersson, Ann ; Nilsson, Linn LU ; Nylund, Gunnar ; Khorram-Manesh, Amir ; Nordgren, Svante and Delbro, Dick (2009) In European Journal of Pharmacology 609(1-3). p.27-33
Abstract
We used immunochemistry to demonstrate expression of acetylcholine's nicotinic α7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine–or speculatively, even intracrine–signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.
Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
European Journal of Pharmacology
volume
609
issue
1-3
pages
27 - 33
publisher
Elsevier
external identifiers
  • scopus:64549105660
ISSN
1879-0712
DOI
10.1016/j.ejphar.2009.03.002
language
English
LU publication?
no
id
a62fa6fe-815d-4699-a7f8-cde6befdeeba
date added to LUP
2024-05-29 15:22:46
date last changed
2024-05-30 07:43:08
@article{a62fa6fe-815d-4699-a7f8-cde6befdeeba,
  abstract     = {{We used immunochemistry to demonstrate expression of acetylcholine's nicotinic α7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine–or speculatively, even intracrine–signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.}},
  author       = {{Pettersson, Ann and Nilsson, Linn and Nylund, Gunnar and Khorram-Manesh, Amir and Nordgren, Svante and Delbro, Dick}},
  issn         = {{1879-0712}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{1-3}},
  pages        = {{27--33}},
  publisher    = {{Elsevier}},
  series       = {{European Journal of Pharmacology}},
  title        = {{Is acetylcholine an autocrine/paracrine growth factor via the nicotinic α7-receptor subtype in the human colon cancer cell line HT-29?}},
  url          = {{http://dx.doi.org/10.1016/j.ejphar.2009.03.002}},
  doi          = {{10.1016/j.ejphar.2009.03.002}},
  volume       = {{609}},
  year         = {{2009}},
}