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Production of an amino acid sequence-specific antiserum against human amyloid A (AA) and serum amyloid A (SAA) protein

Grubb, Anders LU orcid ; Löfberg, Helge LU ; Thysell, Hans LU ; Ljunggren, Lennart ; Olsson, Thomas ; Skinner, Martha ; Shirahama, Tsuranobu and Cohen, Alan S (1987) In Scandinavian Journal of Clinical and Laboratory Investigation 47(6). p.619-626
Abstract

The hydrophilic nonapeptide Ser-Asp-Ala-Arg-Glu-Asn-Ile-Gln-Arg, identical with residues 59-67 of human amyloid protein A (AA) and serum amyloid protein A (SAA), was covalently bound via its carboxyl-terminal end to the carrier-protein keyhole limpet haemocyanin. The complex was injected subcutaneously into ten rabbits. All rabbits produced antisera which, unabsorbed, were specific for AA and SAA. The antisera and their isolated peptide specific antibodies were performance-tested and found to be excellent for demonstration of AA and SAA in immunoblotting and immunohistochemical techniques but unsuitable for immunoprecipitation. Since it is difficult to produce AA- and SAA-specific antisera by procedures earlier described and commercial... (More)

The hydrophilic nonapeptide Ser-Asp-Ala-Arg-Glu-Asn-Ile-Gln-Arg, identical with residues 59-67 of human amyloid protein A (AA) and serum amyloid protein A (SAA), was covalently bound via its carboxyl-terminal end to the carrier-protein keyhole limpet haemocyanin. The complex was injected subcutaneously into ten rabbits. All rabbits produced antisera which, unabsorbed, were specific for AA and SAA. The antisera and their isolated peptide specific antibodies were performance-tested and found to be excellent for demonstration of AA and SAA in immunoblotting and immunohistochemical techniques but unsuitable for immunoprecipitation. Since it is difficult to produce AA- and SAA-specific antisera by procedures earlier described and commercial supplies of good such reagents are unavailable, the easy production of sequence-specific such antisera will facilitate more extended studies of the corresponding antigens for diagnostic and scientific purposes.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Animals, Electrophoresis, Polyacrylamide Gel, Immune Sera/analysis, Immunization, Immunohistochemistry, Molecular Sequence Data, Rabbits, Serum Amyloid A Protein/administration & dosage
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
47
issue
6
pages
8 pages
publisher
Informa Healthcare
external identifiers
  • scopus:0023604620
  • pmid:3672035
ISSN
0036-5513
language
English
LU publication?
yes
id
a6411fa9-ac77-4b8a-bf99-261a73ad3818
alternative location
https://www.tandfonline.com/doi/abs/10.1080/00365518709168478
date added to LUP
2021-10-26 12:04:12
date last changed
2024-01-12 02:56:17
@article{a6411fa9-ac77-4b8a-bf99-261a73ad3818,
  abstract     = {{<p>The hydrophilic nonapeptide Ser-Asp-Ala-Arg-Glu-Asn-Ile-Gln-Arg, identical with residues 59-67 of human amyloid protein A (AA) and serum amyloid protein A (SAA), was covalently bound via its carboxyl-terminal end to the carrier-protein keyhole limpet haemocyanin. The complex was injected subcutaneously into ten rabbits. All rabbits produced antisera which, unabsorbed, were specific for AA and SAA. The antisera and their isolated peptide specific antibodies were performance-tested and found to be excellent for demonstration of AA and SAA in immunoblotting and immunohistochemical techniques but unsuitable for immunoprecipitation. Since it is difficult to produce AA- and SAA-specific antisera by procedures earlier described and commercial supplies of good such reagents are unavailable, the easy production of sequence-specific such antisera will facilitate more extended studies of the corresponding antigens for diagnostic and scientific purposes.</p>}},
  author       = {{Grubb, Anders and Löfberg, Helge and Thysell, Hans and Ljunggren, Lennart and Olsson, Thomas and Skinner, Martha and Shirahama, Tsuranobu and Cohen, Alan S}},
  issn         = {{0036-5513}},
  keywords     = {{Amino Acid Sequence; Animals; Electrophoresis, Polyacrylamide Gel; Immune Sera/analysis; Immunization; Immunohistochemistry; Molecular Sequence Data; Rabbits; Serum Amyloid A Protein/administration & dosage}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{619--626}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Production of an amino acid sequence-specific antiserum against human amyloid A (AA) and serum amyloid A (SAA) protein}},
  url          = {{https://www.tandfonline.com/doi/abs/10.1080/00365518709168478}},
  volume       = {{47}},
  year         = {{1987}},
}