A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27-IgMhigh B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation
(2013) In Clinical Immunology 149(3 Part B). p.421-431- Abstract
The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27+ B cells formed after transplantation with the number of CD27+IgMhigh cells more affected than class-switched ones. A previously unacknowledged population of CD27-IgMhigh cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27-IgMhigh B cells expressed markers typical for transitional B cells, and the non-transitional CD27-IgMhigh cells could be further... (More)
The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27+ B cells formed after transplantation with the number of CD27+IgMhigh cells more affected than class-switched ones. A previously unacknowledged population of CD27-IgMhigh cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27-IgMhigh B cells expressed markers typical for transitional B cells, and the non-transitional CD27-IgMhigh cells could be further divided into subpopulations based on their ability to extrude the dye Rhodamine 123 and their expression of CD45RBMEM55, a glycosylation-dependent epitope. Thus, we define several novel human CD27-IgMhigh B cell subpopulations in blood, all of which are present in higher frequencies and numbers in young children and after HSCT than in adults.
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- author
- Bemark, Mats LU ; Friskopp, Linda ; Saghafian-Hedengren, Shanie ; Koethe, Susanne ; Fasth, Anders ; Abrahamsson, Jonas ; Sverremark-Ekström, Eva ; Andersson, Bengt A. and Mellgren, Karin
- publishing date
- 2013-12
- type
- Contribution to journal
- publication status
- published
- keywords
- B cell, Hematopoietic stem cell transplantation, Immunological ontogeny, Lymphocyte development
- in
- Clinical Immunology
- volume
- 149
- issue
- 3 Part B
- pages
- 421 - 431
- publisher
- Elsevier
- external identifiers
-
- pmid:24211716
- scopus:84887151419
- ISSN
- 1521-6616
- DOI
- 10.1016/j.clim.2013.08.011
- language
- English
- LU publication?
- no
- id
- a67dbacc-410e-4caf-9b21-d0503994adc0
- date added to LUP
- 2023-12-06 17:09:51
- date last changed
- 2024-10-05 04:57:52
@article{a67dbacc-410e-4caf-9b21-d0503994adc0, abstract = {{<p>The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27<sup>+</sup> B cells formed after transplantation with the number of CD27<sup>+</sup>IgM<sup>high</sup> cells more affected than class-switched ones. A previously unacknowledged population of CD27<sup>-</sup>IgM<sup>high</sup> cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27<sup>-</sup>IgM<sup>high</sup> B cells expressed markers typical for transitional B cells, and the non-transitional CD27<sup>-</sup>IgM<sup>high</sup> cells could be further divided into subpopulations based on their ability to extrude the dye Rhodamine 123 and their expression of CD45RB<sup>MEM55</sup>, a glycosylation-dependent epitope. Thus, we define several novel human CD27<sup>-</sup>IgM<sup>high</sup> B cell subpopulations in blood, all of which are present in higher frequencies and numbers in young children and after HSCT than in adults.</p>}}, author = {{Bemark, Mats and Friskopp, Linda and Saghafian-Hedengren, Shanie and Koethe, Susanne and Fasth, Anders and Abrahamsson, Jonas and Sverremark-Ekström, Eva and Andersson, Bengt A. and Mellgren, Karin}}, issn = {{1521-6616}}, keywords = {{B cell; Hematopoietic stem cell transplantation; Immunological ontogeny; Lymphocyte development}}, language = {{eng}}, number = {{3 Part B}}, pages = {{421--431}}, publisher = {{Elsevier}}, series = {{Clinical Immunology}}, title = {{A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27<sup>-</sup>IgM<sup>high</sup> B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation}}, url = {{http://dx.doi.org/10.1016/j.clim.2013.08.011}}, doi = {{10.1016/j.clim.2013.08.011}}, volume = {{149}}, year = {{2013}}, }