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Development of a miniaturized glucose monitor for whole blood measurements

Hedberg, U. ; Xie, B. LU and Danielsson, B. LU (1992) p.49-54
Abstract
The glucose concentration in diluted whole blood has been measured, using a miniaturized thermal biosensor based on the enzyme thermistor principle. The biosensor is a small flow-injection system. A sample volume of 20μ1 is injected into a flow of 50μl/min. The heat produced when the sample passes the enzyme column is measured with a thermistor connected to a Wheatstone bridge. The enzyme column contains glucose oxidase and catalase co-immobilized on a solid support material. Samples of whole blood usually cause problems in flowsystems. The blood cells tend to block the enzyme column and the back pressure increases. We have tested a superporous agarose material as enzyme support material using ten-fold diluted samples of whole human blood.... (More)
The glucose concentration in diluted whole blood has been measured, using a miniaturized thermal biosensor based on the enzyme thermistor principle. The biosensor is a small flow-injection system. A sample volume of 20μ1 is injected into a flow of 50μl/min. The heat produced when the sample passes the enzyme column is measured with a thermistor connected to a Wheatstone bridge. The enzyme column contains glucose oxidase and catalase co-immobilized on a solid support material. Samples of whole blood usually cause problems in flowsystems. The blood cells tend to block the enzyme column and the back pressure increases. We have tested a superporous agarose material as enzyme support material using ten-fold diluted samples of whole human blood. The blood was collected from the finger-tip and diluted with buffer containing an anticoagulant and sodium fluoride. The number of samples possible to inject and the accuracy compared to the Boehringer Mannheim Reflolux have been determined. At least 100 ten-fold diluted blood samples could be injected on a micro-column of superporous agarose. The obtained glucose concentration correlated well with the one obtained with the Boehringer Mannheim Reflolux. (Less)
Please use this url to cite or link to this publication:
author
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Biosensors '92 Proceedings : The Second World Congress on Biosensors - The Second World Congress on Biosensors
editor
Heineman, W. R. ; Karube, I. ; Schmid, R. D. and Turner, A. P. F.
pages
6 pages
publisher
Elsevier
ISBN
978-1-85617-161-8
DOI
10.1016/B978-1-85617-161-8.50011-2
language
English
LU publication?
yes
id
a8b20aeb-3897-4d83-9daf-4465009405e0
alternative location
https://books.google.se/books?hl=en&lr=&id=N3KeBQAAQBAJ&oi=fnd&pg=PA49&ots=r0t8hd102k&sig=f9vjS30SkDSTkfLBsPOmULATtY8&redir_esc=y#v=onepage&q&f=false
date added to LUP
2021-10-27 11:27:19
date last changed
2021-10-29 11:59:36
@inproceedings{a8b20aeb-3897-4d83-9daf-4465009405e0,
  abstract     = {{The glucose concentration in diluted whole blood has been measured, using a miniaturized thermal biosensor based on the enzyme thermistor principle. The biosensor is a small flow-injection system. A sample volume of 20μ1 is injected into a flow of 50μl/min. The heat produced when the sample passes the enzyme column is measured with a thermistor connected to a Wheatstone bridge. The enzyme column contains glucose oxidase and catalase co-immobilized on a solid support material. Samples of whole blood usually cause problems in flowsystems. The blood cells tend to block the enzyme column and the back pressure increases. We have tested a superporous agarose material as enzyme support material using ten-fold diluted samples of whole human blood. The blood was collected from the finger-tip and diluted with buffer containing an anticoagulant and sodium fluoride. The number of samples possible to inject and the accuracy compared to the Boehringer Mannheim Reflolux have been determined. At least 100 ten-fold diluted blood samples could be injected on a micro-column of superporous agarose. The obtained glucose concentration correlated well with the one obtained with the Boehringer Mannheim Reflolux.}},
  author       = {{Hedberg, U. and Xie, B. and Danielsson, B.}},
  booktitle    = {{Biosensors '92 Proceedings : The Second World Congress on Biosensors}},
  editor       = {{Heineman, W. R. and Karube, I. and Schmid, R. D. and Turner, A. P. F.}},
  isbn         = {{978-1-85617-161-8}},
  language     = {{eng}},
  pages        = {{49--54}},
  publisher    = {{Elsevier}},
  title        = {{Development of a miniaturized glucose monitor for whole blood measurements}},
  url          = {{http://dx.doi.org/10.1016/B978-1-85617-161-8.50011-2}},
  doi          = {{10.1016/B978-1-85617-161-8.50011-2}},
  year         = {{1992}},
}