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Characterization of human aquaporin protein-protein interactions using microscale thermophoresis (MST)

Al-Jubair, Tamim LU ; Steffen, Jonas Hyld ; Missel, Julie Winkel ; Kitchen, Philip ; Salman, Mootaz M. ; Bill, Roslyn M. ; Gourdon, Pontus LU and Törnroth-Horsefield, Susanna LU (2022) In STAR Protocols 3(2).
Abstract

Aquaporin water channels (AQPs) are membrane proteins that maintain cellular water homeostasis. The interactions between human AQPs and other proteins play crucial roles in AQP regulation by both gating and trafficking. Here, we describe a protocol for characterizing the interaction between a human AQP and a soluble interaction partner using microscale thermophoresis (MST). MST has the advantage of low sample consumption and high detergent compatibility enabling AQP protein-protein interaction investigation with a high level of control of components and environment. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Roche et al. (2017).

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Membrane, Protein Biochemistry, Single-molecule Assays
in
STAR Protocols
volume
3
issue
2
article number
101316
publisher
Cell Press
external identifiers
  • pmid:35479114
  • scopus:85128271354
ISSN
2666-1667
DOI
10.1016/j.xpro.2022.101316
language
English
LU publication?
yes
id
aa058cfd-37e9-405a-9e1f-6189e48aa825
date added to LUP
2022-06-17 15:57:42
date last changed
2025-01-24 17:14:57
@article{aa058cfd-37e9-405a-9e1f-6189e48aa825,
  abstract     = {{<p>Aquaporin water channels (AQPs) are membrane proteins that maintain cellular water homeostasis. The interactions between human AQPs and other proteins play crucial roles in AQP regulation by both gating and trafficking. Here, we describe a protocol for characterizing the interaction between a human AQP and a soluble interaction partner using microscale thermophoresis (MST). MST has the advantage of low sample consumption and high detergent compatibility enabling AQP protein-protein interaction investigation with a high level of control of components and environment. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Roche et al. (2017).</p>}},
  author       = {{Al-Jubair, Tamim and Steffen, Jonas Hyld and Missel, Julie Winkel and Kitchen, Philip and Salman, Mootaz M. and Bill, Roslyn M. and Gourdon, Pontus and Törnroth-Horsefield, Susanna}},
  issn         = {{2666-1667}},
  keywords     = {{Cell Membrane; Protein Biochemistry; Single-molecule Assays}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{2}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Characterization of human aquaporin protein-protein interactions using microscale thermophoresis (MST)}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2022.101316}},
  doi          = {{10.1016/j.xpro.2022.101316}},
  volume       = {{3}},
  year         = {{2022}},
}