Molecularly imprinted microspheres as antibody binding mimics
(2001) 9th International Conference on Polymer-Based Technology In Reactive and Functional Polymers 48(1-3). p.149-157- Abstract
Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of... (More)
Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.
(Less)
- author
- Ye, Lei LU and Mosbach, Klaus LU
- organization
- publishing date
- 2001-05-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enzyme-linked immunosorbent assay, Immunoassay, Microspheres, Molecular imprinting, Precipitation polymerization
- in
- Reactive and Functional Polymers
- volume
- 48
- issue
- 1-3
- pages
- 9 pages
- publisher
- Elsevier
- conference name
- 9th International Conference on Polymer-Based Technology
- conference location
- China
- conference dates
- 2000-05-21 - 2000-05-21
- external identifiers
-
- scopus:0035795570
- ISSN
- 1381-5148
- DOI
- 10.1016/S1381-5148(01)00050-5
- language
- English
- LU publication?
- yes
- id
- aa07a0a4-ac38-4d3d-9391-227f44e6959b
- date added to LUP
- 2019-06-18 09:00:00
- date last changed
- 2022-03-03 00:25:42
@article{aa07a0a4-ac38-4d3d-9391-227f44e6959b, abstract = {{<p>Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.</p>}}, author = {{Ye, Lei and Mosbach, Klaus}}, issn = {{1381-5148}}, keywords = {{Enzyme-linked immunosorbent assay; Immunoassay; Microspheres; Molecular imprinting; Precipitation polymerization}}, language = {{eng}}, month = {{05}}, number = {{1-3}}, pages = {{149--157}}, publisher = {{Elsevier}}, series = {{Reactive and Functional Polymers}}, title = {{Molecularly imprinted microspheres as antibody binding mimics}}, url = {{http://dx.doi.org/10.1016/S1381-5148(01)00050-5}}, doi = {{10.1016/S1381-5148(01)00050-5}}, volume = {{48}}, year = {{2001}}, }