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Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase

Fu, Tian-Min ; Li, Dan ; Nan, Jie LU ; Li, Lanfen ; Xue, Yafeng and Su, Xiao-Dong LU (2007) In Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 63(Pt 12). p.8-1026
Abstract

As a substrate of extracellular signal-related kinase (ERK), the p90 ribosome S6 kinase 1 (RSK1) is at the terminus of the Ras/ERK pathway. Residues 411-735 of human RSK1, covering the C-terminal serine/threonine kinase catalytic domain and the functionally important tail, were cloned into an Escherichia coli expression vector. The protein was expressed, purified and crystallized. The crystals diffracted to 2.7 A and belonged to space group P2(1), with unit-cell parameters a = 39.8, b = 143.8, c = 59.9 A, beta = 95.7 degrees.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Crystallization, Crystallography, X-Ray, Humans, Protein-Serine-Threonine Kinases, Ribosomal Protein S6 Kinases, 90-kDa
in
Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
volume
63
issue
Pt 12
pages
3 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:36849085969
  • pmid:18084084
ISSN
2053-230X
DOI
10.1107/S1744309107051329
language
English
LU publication?
yes
id
ac1dbddd-5583-4042-a39e-8c9e1673f595
date added to LUP
2016-09-07 22:53:56
date last changed
2024-01-04 12:07:19
@article{ac1dbddd-5583-4042-a39e-8c9e1673f595,
  abstract     = {{<p>As a substrate of extracellular signal-related kinase (ERK), the p90 ribosome S6 kinase 1 (RSK1) is at the terminus of the Ras/ERK pathway. Residues 411-735 of human RSK1, covering the C-terminal serine/threonine kinase catalytic domain and the functionally important tail, were cloned into an Escherichia coli expression vector. The protein was expressed, purified and crystallized. The crystals diffracted to 2.7 A and belonged to space group P2(1), with unit-cell parameters a = 39.8, b = 143.8, c = 59.9 A, beta = 95.7 degrees.</p>}},
  author       = {{Fu, Tian-Min and Li, Dan and Nan, Jie and Li, Lanfen and Xue, Yafeng and Su, Xiao-Dong}},
  issn         = {{2053-230X}},
  keywords     = {{Crystallization; Crystallography, X-Ray; Humans; Protein-Serine-Threonine Kinases; Ribosomal Protein S6 Kinases, 90-kDa}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{Pt 12}},
  pages        = {{8--1026}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Acta Crystallographica. Section F: Structural Biology and Crystallization Communications}},
  title        = {{Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase}},
  url          = {{http://dx.doi.org/10.1107/S1744309107051329}},
  doi          = {{10.1107/S1744309107051329}},
  volume       = {{63}},
  year         = {{2007}},
}