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Centrosome Movements Are TUBG1-Dependent

Malycheva, Darina LU and Alvarado Kristensson, Maria LU (2023) In International Journal of Molecular Sciences 24(17). p.1-11
Abstract
The centrosome of mammalian cells is in constant movement and its motion plays a part in cell differentiation and cell division. The purpose of this study was to establish the involvement of the TUBG meshwork in centrosomal motility. In live cells, we used a monomeric red-fluorescenceprotein-tagged centrin 2 gene and a green-fluorescence-protein-tagged TUBG1 gene for labeling the centrosome and the TUBG1 meshwork, respectively. We found that centrosome movements occurred
in cellular sites rich in GTPase TUBG1 and single-guide RNA mediated a reduction in the expression of TUBG1, altering the motility pattern of centrosomes. We propose that the TUBG1 meshwork enables the centrosomes to move by providing them with an interacting platform... (More)
The centrosome of mammalian cells is in constant movement and its motion plays a part in cell differentiation and cell division. The purpose of this study was to establish the involvement of the TUBG meshwork in centrosomal motility. In live cells, we used a monomeric red-fluorescenceprotein-tagged centrin 2 gene and a green-fluorescence-protein-tagged TUBG1 gene for labeling the centrosome and the TUBG1 meshwork, respectively. We found that centrosome movements occurred
in cellular sites rich in GTPase TUBG1 and single-guide RNA mediated a reduction in the expression of TUBG1, altering the motility pattern of centrosomes. We propose that the TUBG1 meshwork enables the centrosomes to move by providing them with an interacting platform that mediates positional changes. These findings uncover a novel regulatory mechanism that controls the behavior of centrosomes. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Molecular Sciences
volume
24
issue
17
article number
13154
pages
1 - 11
publisher
MDPI AG
external identifiers
  • scopus:85170206856
ISSN
1422-0067
DOI
10.3390/ijms241713154
language
English
LU publication?
yes
id
b2973177-5347-4541-82c8-9de47167f430
date added to LUP
2023-08-23 21:12:19
date last changed
2025-04-04 15:00:55
@article{b2973177-5347-4541-82c8-9de47167f430,
  abstract     = {{The centrosome of mammalian cells is in constant movement and its motion plays a part in cell differentiation and cell division. The purpose of this study was to establish the involvement of the TUBG meshwork in centrosomal motility. In live cells, we used a monomeric red-fluorescenceprotein-tagged centrin 2 gene and a green-fluorescence-protein-tagged TUBG1 gene for labeling the centrosome and the TUBG1 meshwork, respectively. We found that centrosome movements occurred<br/>in cellular sites rich in GTPase TUBG1 and single-guide RNA mediated a reduction in the expression of TUBG1, altering the motility pattern of centrosomes. We propose that the TUBG1 meshwork enables the centrosomes to move by providing them with an interacting platform that mediates positional changes. These findings uncover a novel regulatory mechanism that controls the behavior of centrosomes.}},
  author       = {{Malycheva, Darina and Alvarado Kristensson, Maria}},
  issn         = {{1422-0067}},
  language     = {{eng}},
  number       = {{17}},
  pages        = {{1--11}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Centrosome Movements Are TUBG1-Dependent}},
  url          = {{https://lup.lub.lu.se/search/files/155708116/ijms_24_13154.pdf}},
  doi          = {{10.3390/ijms241713154}},
  volume       = {{24}},
  year         = {{2023}},
}