Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Functionalized synchrotron in-line phase-contrast computed tomography : A novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples

Dullin, Christian ; Dal Monego, Simeone ; Larsson, Emanuel LU orcid ; Mohammadi, Sara ; Krenkel, Martin ; Garrovo, Chiara ; Biffi, Stefania ; Lorenzon, Andrea ; Markus, Andrea and Napp, Joanna , et al. (2015) In Journal of Synchrotron Radiation 22(1). p.143-155
Abstract

Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline... (More)

Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
keywords
functional CT imaging, phase-contrast CT, single-distance phase retrieval
in
Journal of Synchrotron Radiation
volume
22
issue
1
pages
143 - 155
publisher
International Union of Crystallography
external identifiers
  • scopus:84919968656
  • pmid:25537601
ISSN
0909-0495
DOI
10.1107/S1600577514021730
language
English
LU publication?
no
additional info
Publisher Copyright: © 2015 International Union of Crystallography.
id
b30d785e-a5b3-4d3d-8b3d-cec4d229440b
date added to LUP
2025-08-26 11:23:41
date last changed
2025-08-27 03:24:52
@article{b30d785e-a5b3-4d3d-8b3d-cec4d229440b,
  abstract     = {{<p>Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24h, lungs were kept in situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites.</p>}},
  author       = {{Dullin, Christian and Dal Monego, Simeone and Larsson, Emanuel and Mohammadi, Sara and Krenkel, Martin and Garrovo, Chiara and Biffi, Stefania and Lorenzon, Andrea and Markus, Andrea and Napp, Joanna and Salditt, Tim and Accardo, Agostino and Alves, Frauke and Tromba, Giuliana}},
  issn         = {{0909-0495}},
  keywords     = {{functional CT imaging; phase-contrast CT; single-distance phase retrieval}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{143--155}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{Functionalized synchrotron in-line phase-contrast computed tomography : A novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples}},
  url          = {{http://dx.doi.org/10.1107/S1600577514021730}},
  doi          = {{10.1107/S1600577514021730}},
  volume       = {{22}},
  year         = {{2015}},
}