Effect of recombinant neutral endopeptidase (EC 3.4.24.11) on neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat
(1998) In American Journal of Rhinology 12(2). p.41-135- Abstract
The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and... (More)
The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat, we suggest that peptidase activity in the nasal mucosa will determine the magnitude of the response to locally released neuropeptides.
(Less)
- author
- Josefsson, Malin LU ; Becker, Magnus LU ; Stroman, F ; Brenner, Daniel G and Petersson, Göran
- publishing date
- 1998-05-14
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Administration, Topical, Animals, Capsaicin/pharmacology, Exudates and Transudates/metabolism, Male, Nasal Mucosa/drug effects, Neprilysin/pharmacology, Neuropeptides/physiology, Plasma/metabolism, Rats, Rats, Sprague-Dawley, Recombinant Proteins, Substance P/pharmacology
- in
- American Journal of Rhinology
- volume
- 12
- issue
- 2
- pages
- 7 pages
- publisher
- OceanSide Publications, Inc
- external identifiers
-
- scopus:0031981760
- pmid:9578933
- ISSN
- 1050-6586
- DOI
- 10.2500/105065898781390244
- language
- English
- LU publication?
- no
- id
- b35e3878-1086-4993-9612-1dab555e5649
- date added to LUP
- 2021-04-19 16:37:56
- date last changed
- 2024-10-05 22:37:12
@article{b35e3878-1086-4993-9612-1dab555e5649, abstract = {{<p>The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat, we suggest that peptidase activity in the nasal mucosa will determine the magnitude of the response to locally released neuropeptides.</p>}}, author = {{Josefsson, Malin and Becker, Magnus and Stroman, F and Brenner, Daniel G and Petersson, Göran}}, issn = {{1050-6586}}, keywords = {{Administration, Topical; Animals; Capsaicin/pharmacology; Exudates and Transudates/metabolism; Male; Nasal Mucosa/drug effects; Neprilysin/pharmacology; Neuropeptides/physiology; Plasma/metabolism; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Substance P/pharmacology}}, language = {{eng}}, month = {{05}}, number = {{2}}, pages = {{41--135}}, publisher = {{OceanSide Publications, Inc}}, series = {{American Journal of Rhinology}}, title = {{Effect of recombinant neutral endopeptidase (EC 3.4.24.11) on neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat}}, url = {{http://dx.doi.org/10.2500/105065898781390244}}, doi = {{10.2500/105065898781390244}}, volume = {{12}}, year = {{1998}}, }