Rapid detection of ciguatoxins in Gambierdiscus and Fukuyoa with immunosensing tools
(2020) In Ecotoxicology and Environmental Safety 204.- Abstract
Consumption of seafood contaminated with ciguatoxins (CTXs) leads to a foodborne disease known as ciguatera. Primary producers of CTXs are epibenthic dinoflagellates of the genera Gambierdiscus and Fukuyoa. In this study, thirteen Gambierdiscus and Fukuyoa strains were cultured, harvested at exponential phase, and CTXs were extracted with an implemented rapid protocol. Microalgal extracts were obtained from pellets with a low cell abundance (20,000 cell/mL) and were then analyzed with magnetic bead (MB)-based immunosensing tools (colorimetric immunoassay and electrochemical immunosensor). It is the first time that these approaches are used to screen Gambierdiscus and Fukuyoa strains, providing not only a global indication of the... (More)
Consumption of seafood contaminated with ciguatoxins (CTXs) leads to a foodborne disease known as ciguatera. Primary producers of CTXs are epibenthic dinoflagellates of the genera Gambierdiscus and Fukuyoa. In this study, thirteen Gambierdiscus and Fukuyoa strains were cultured, harvested at exponential phase, and CTXs were extracted with an implemented rapid protocol. Microalgal extracts were obtained from pellets with a low cell abundance (20,000 cell/mL) and were then analyzed with magnetic bead (MB)-based immunosensing tools (colorimetric immunoassay and electrochemical immunosensor). It is the first time that these approaches are used to screen Gambierdiscus and Fukuyoa strains, providing not only a global indication of the presence of CTXs, but also the ability to discriminate between two series of congeners (CTX1B and CTX3C). Analysis of the microalgal extracts revealed the presence of CTXs in 11 out of 13 strains and provided new information about Gambierdiscus and Fukuyoa toxin profiles. The use of immunosensing tools in the analysis of microalgal extracts facilitates the elucidation of further knowledge regarding these dinoflagellate genera and can contribute to improved ciguatera risk assessment and management.
(Less)
- author
- publishing date
- 2020-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Ciguatera, Ciguatoxins (CTXs), Fukuyoa, Gambierdiscus, Immunoassay, Immunosensor
- in
- Ecotoxicology and Environmental Safety
- volume
- 204
- article number
- 111004
- publisher
- Elsevier
- external identifiers
-
- pmid:32768745
- scopus:85089007404
- ISSN
- 0147-6513
- DOI
- 10.1016/j.ecoenv.2020.111004
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2020 Elsevier Inc.
- id
- b3de02b1-bd5e-46c2-a7a1-b0a7643558ed
- date added to LUP
- 2024-07-09 13:13:03
- date last changed
- 2024-09-03 19:44:55
@article{b3de02b1-bd5e-46c2-a7a1-b0a7643558ed,
abstract = {{<p>Consumption of seafood contaminated with ciguatoxins (CTXs) leads to a foodborne disease known as ciguatera. Primary producers of CTXs are epibenthic dinoflagellates of the genera Gambierdiscus and Fukuyoa. In this study, thirteen Gambierdiscus and Fukuyoa strains were cultured, harvested at exponential phase, and CTXs were extracted with an implemented rapid protocol. Microalgal extracts were obtained from pellets with a low cell abundance (20,000 cell/mL) and were then analyzed with magnetic bead (MB)-based immunosensing tools (colorimetric immunoassay and electrochemical immunosensor). It is the first time that these approaches are used to screen Gambierdiscus and Fukuyoa strains, providing not only a global indication of the presence of CTXs, but also the ability to discriminate between two series of congeners (CTX1B and CTX3C). Analysis of the microalgal extracts revealed the presence of CTXs in 11 out of 13 strains and provided new information about Gambierdiscus and Fukuyoa toxin profiles. The use of immunosensing tools in the analysis of microalgal extracts facilitates the elucidation of further knowledge regarding these dinoflagellate genera and can contribute to improved ciguatera risk assessment and management.</p>}},
author = {{Gaiani, G. and Leonardo, S. and Tudó and Toldrà, A. and Rey, M. and Andree, K. B. and Tsumuraya, T. and Hirama, M. and Diogène, J. and O'Sullivan, C. K. and Alcaraz, C. and Campàs, M.}},
issn = {{0147-6513}},
keywords = {{Ciguatera; Ciguatoxins (CTXs); Fukuyoa; Gambierdiscus; Immunoassay; Immunosensor}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Ecotoxicology and Environmental Safety}},
title = {{Rapid detection of ciguatoxins in Gambierdiscus and Fukuyoa with immunosensing tools}},
url = {{http://dx.doi.org/10.1016/j.ecoenv.2020.111004}},
doi = {{10.1016/j.ecoenv.2020.111004}},
volume = {{204}},
year = {{2020}},
}