Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures
Estévez-Priego, Estefanía ; Moreno-Fina, Martina ; Monni, Emanuela LU ; Kokaia, Zaal LU
; Soriano, Jordi
and Tornero, Daniel
(2023)
In Stem Cell Reports
18(1).
p.205-219
- Abstract
Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in... (More)
Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration.
(Less)
- author
- Estévez-Priego, Estefanía
; Moreno-Fina, Martina
; Monni, Emanuela
LU
; Kokaia, Zaal
LU
; Soriano, Jordi
and Tornero, Daniel
- organization
- publishing date
- 2023-01-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- calcium imaging, functional connectivity, human cellular models, human iPSCs, neural stem cells, neuronal networks
- in
- Stem Cell Reports
- volume
- 18
- issue
- 1
- pages
- 15 pages
- publisher
- Cell Press
- external identifiers
-
- pmid:36563684
- scopus:85146065115
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2022.11.014
- language
- English
- LU publication?
- yes
- id
- b41ef3b9-1f91-473b-9045-826169a8643f
- date added to LUP
- 2023-02-17 13:18:09
- date last changed
- 2025-02-05 00:12:13
@article{b41ef3b9-1f91-473b-9045-826169a8643f,
abstract = {{<p>Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration.</p>}},
author = {{Estévez-Priego, Estefanía and Moreno-Fina, Martina and Monni, Emanuela and Kokaia, Zaal and Soriano, Jordi and Tornero, Daniel}},
issn = {{2213-6711}},
keywords = {{calcium imaging; functional connectivity; human cellular models; human iPSCs; neural stem cells; neuronal networks}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{205--219}},
publisher = {{Cell Press}},
series = {{Stem Cell Reports}},
title = {{Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures}},
url = {{http://dx.doi.org/10.1016/j.stemcr.2022.11.014}},
doi = {{10.1016/j.stemcr.2022.11.014}},
volume = {{18}},
year = {{2023}},
}