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Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation

Baba, Hideo A ; Wohlschlaeger, Jeremias ; Schmitz, Klaus J ; Nadalin, Silvio ; Lang, Hauke ; Benesch, Alexandra ; Gu, Yanli ; Biglarnia, Ali-R LU orcid ; Sotiropoulos, Georgios C and Takeda, Atsushi , et al. (2009) In Liver International 29(4). p.92-585
Abstract

BACKGROUND: Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.

METHODS: Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as... (More)

BACKGROUND: Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.

METHODS: Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as controls.

RESULTS: Survivin transcript and protein were significantly upregulated in rat hepatocytes after 24-72 h during regeneration and showed a significant correlation with proliferation but not with apoptosis. In humans, survivin was nearly absent in donor and reperfused liver tissue but increased significantly 5-7 days after transplantation and correlated with proliferation but not with apoptosis.

CONCLUSIONS: Survivin is upregulated in human and rodent liver regeneration and correlates with proliferation, suggesting an association of survivin and cell division.

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publishing date
type
Contribution to journal
publication status
published
keywords
Adult, Animals, Apoptosis, Biopsy, Cell Proliferation, Female, Gene Expression, Hepatectomy/methods, Hepatocytes/cytology, Humans, Inhibitor of Apoptosis Proteins, Liver/metabolism, Liver Regeneration/physiology, Liver Transplantation, Male, Microtubule-Associated Proteins/genetics, Middle Aged, RNA, Messenger/metabolism, Rats, Rats, Inbred Lew, Survivin, Up-Regulation
in
Liver International
volume
29
issue
4
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:62749123474
  • pmid:19018973
ISSN
1478-3231
DOI
10.1111/j.1478-3231.2008.01911.x
language
English
LU publication?
no
id
b4a8c929-05c2-40ca-8045-46be6d468277
date added to LUP
2025-12-17 14:21:56
date last changed
2025-12-19 02:25:41
@article{b4a8c929-05c2-40ca-8045-46be6d468277,
  abstract     = {{<p>BACKGROUND: Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans.</p><p>METHODS: Seventy per cent liver resection was used to investigate liver regeneration in rats. Survivin was identified by means of reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Proliferation and apoptosis were quantified. Liver biopsies from 33 patients who underwent living donor liver transplantation were used to study survivin immuno-expression, proliferation and apoptosis within the first 17 days after transplantation. Seven healthy donors served as controls.</p><p>RESULTS: Survivin transcript and protein were significantly upregulated in rat hepatocytes after 24-72 h during regeneration and showed a significant correlation with proliferation but not with apoptosis. In humans, survivin was nearly absent in donor and reperfused liver tissue but increased significantly 5-7 days after transplantation and correlated with proliferation but not with apoptosis.</p><p>CONCLUSIONS: Survivin is upregulated in human and rodent liver regeneration and correlates with proliferation, suggesting an association of survivin and cell division.</p>}},
  author       = {{Baba, Hideo A and Wohlschlaeger, Jeremias and Schmitz, Klaus J and Nadalin, Silvio and Lang, Hauke and Benesch, Alexandra and Gu, Yanli and Biglarnia, Ali-R and Sotiropoulos, Georgios C and Takeda, Atsushi and Takeda, Nobuakira and von Wnuck Lipinski, Karen and Levkau, Bodo}},
  issn         = {{1478-3231}},
  keywords     = {{Adult; Animals; Apoptosis; Biopsy; Cell Proliferation; Female; Gene Expression; Hepatectomy/methods; Hepatocytes/cytology; Humans; Inhibitor of Apoptosis Proteins; Liver/metabolism; Liver Regeneration/physiology; Liver Transplantation; Male; Microtubule-Associated Proteins/genetics; Middle Aged; RNA, Messenger/metabolism; Rats; Rats, Inbred Lew; Survivin; Up-Regulation}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{92--585}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Liver International}},
  title        = {{Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation}},
  url          = {{http://dx.doi.org/10.1111/j.1478-3231.2008.01911.x}},
  doi          = {{10.1111/j.1478-3231.2008.01911.x}},
  volume       = {{29}},
  year         = {{2009}},
}