Increased proportion of CD8+ tumor responsive T cells after immunization with tum- versus tum+ rat glioma
(1995) In Cellular Immunology 165(2). p.33-225- Abstract
Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte... (More)
Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte dominance after tum+ immunization. Although the original tum+ N32 tumor cells are not capable of inducing a clearly demonstrable isograft rejection response, they induce a strong T cell response readily detectable in MLTC assays. We propose that the increased CD8+ lymphocyte proliferation could be an essential feature of the isograft rejection response induced by tum- tumor variants. Possible mechanisms of the augmented CD8+ T cell response are discussed.
(Less)
- author
- Siesjö, P LU ; Visse, E LU and Sjögren, H O LU
- organization
- publishing date
- 1995-10-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, CD8-Positive T-Lymphocytes/immunology, Flow Cytometry, Glioma/immunology, Graft Rejection, Immunization, Lymphocyte Activation, Rats, Rats, Inbred F344, Transplantation, Isogeneic, Tumor Cells, Cultured
- in
- Cellular Immunology
- volume
- 165
- issue
- 2
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0028847190
- pmid:7553887
- ISSN
- 0008-8749
- DOI
- 10.1006/cimm.1995.1209
- language
- English
- LU publication?
- yes
- id
- b54ce117-d0fd-47f8-b361-94b36a5e5412
- date added to LUP
- 2019-06-27 10:22:00
- date last changed
- 2024-01-01 13:37:26
@article{b54ce117-d0fd-47f8-b361-94b36a5e5412, abstract = {{<p>Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte dominance after tum+ immunization. Although the original tum+ N32 tumor cells are not capable of inducing a clearly demonstrable isograft rejection response, they induce a strong T cell response readily detectable in MLTC assays. We propose that the increased CD8+ lymphocyte proliferation could be an essential feature of the isograft rejection response induced by tum- tumor variants. Possible mechanisms of the augmented CD8+ T cell response are discussed.</p>}}, author = {{Siesjö, P and Visse, E and Sjögren, H O}}, issn = {{0008-8749}}, keywords = {{Animals; CD8-Positive T-Lymphocytes/immunology; Flow Cytometry; Glioma/immunology; Graft Rejection; Immunization; Lymphocyte Activation; Rats; Rats, Inbred F344; Transplantation, Isogeneic; Tumor Cells, Cultured}}, language = {{eng}}, month = {{10}}, number = {{2}}, pages = {{33--225}}, publisher = {{Elsevier}}, series = {{Cellular Immunology}}, title = {{Increased proportion of CD8+ tumor responsive T cells after immunization with tum- versus tum+ rat glioma}}, url = {{http://dx.doi.org/10.1006/cimm.1995.1209}}, doi = {{10.1006/cimm.1995.1209}}, volume = {{165}}, year = {{1995}}, }