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Malvidin and cyanidin derivatives from açai fruit (Euterpe oleracea Mart.) counteract UV-A-induced oxidative stress in immortalized fibroblasts

Petruk, Ganna LU orcid ; Illiano, Anna ; Del Giudice, Rita LU ; Raiola, Assunta ; Amoresano, Angela ; Rigano, Maria Manuela ; Piccoli, Renata and Monti, Daria Maria (2017) In Journal of Photochemistry and Photobiology B: Biology 172. p.42-51
Abstract

UV-A radiations are known to induce cellular oxidative stress, leading to premature skin aging. Consumption of açai fruit (Euterpe oleracea Martius) is known to have many health benefits due to its high level of antioxidants. Herein, we analyzed the ability of phenolic compounds extracted from this fruit to attenuate UV-A-induced oxidative stress in immortalized fibroblast. A methanol/water açai extract was fractionated by HPLC and each fraction tested for anti-oxidant stress activity. Immortalized fibroblasts were pre-incubated with açai fractions and then exposed to UV-A radiations. Açai extract was found to be able to strongly protect cells from oxidative stress. In particular, reactive oxygen species (ROS) production, GSH depletion,... (More)

UV-A radiations are known to induce cellular oxidative stress, leading to premature skin aging. Consumption of açai fruit (Euterpe oleracea Martius) is known to have many health benefits due to its high level of antioxidants. Herein, we analyzed the ability of phenolic compounds extracted from this fruit to attenuate UV-A-induced oxidative stress in immortalized fibroblast. A methanol/water açai extract was fractionated by HPLC and each fraction tested for anti-oxidant stress activity. Immortalized fibroblasts were pre-incubated with açai fractions and then exposed to UV-A radiations. Açai extract was found to be able to strongly protect cells from oxidative stress. In particular, reactive oxygen species (ROS) production, GSH depletion, lipid peroxidation and no increase in the phosphorylation levels of proteins involved in the oxidative stress pathway was observed in cells pre-incubated with the extract and then irradiated by UV-A. Mass spectrometry analyses of HPLC fractionated extract led us to the identification of malvidin and cyanidin derivatives as the most active molecules able to counteract the negative effects induced by UV-A irradiation. Our results indicate, for the first time, that açai fruit is a valuable natural source for malvidin and cyanidin to be used as anti-stress molecules and represent good candidates for dietary intervention in the prevention of age related skin damage.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Antioxidants, Eukaryotic cells, Euterpe oleracea, Malvidin, Oxidative stress, Phenolics, UV-A radiations
in
Journal of Photochemistry and Photobiology B: Biology
volume
172
pages
42 - 51
publisher
Elsevier
external identifiers
  • scopus:85019181869
  • pmid:28527426
ISSN
1011-1344
DOI
10.1016/j.jphotobiol.2017.05.013
language
English
LU publication?
no
additional info
Publisher Copyright: © 2017 Elsevier B.V.
id
b8c868c7-d835-4223-9636-1c9acdb565ec
date added to LUP
2025-01-21 15:45:36
date last changed
2025-07-09 16:37:07
@article{b8c868c7-d835-4223-9636-1c9acdb565ec,
  abstract     = {{<p>UV-A radiations are known to induce cellular oxidative stress, leading to premature skin aging. Consumption of açai fruit (Euterpe oleracea Martius) is known to have many health benefits due to its high level of antioxidants. Herein, we analyzed the ability of phenolic compounds extracted from this fruit to attenuate UV-A-induced oxidative stress in immortalized fibroblast. A methanol/water açai extract was fractionated by HPLC and each fraction tested for anti-oxidant stress activity. Immortalized fibroblasts were pre-incubated with açai fractions and then exposed to UV-A radiations. Açai extract was found to be able to strongly protect cells from oxidative stress. In particular, reactive oxygen species (ROS) production, GSH depletion, lipid peroxidation and no increase in the phosphorylation levels of proteins involved in the oxidative stress pathway was observed in cells pre-incubated with the extract and then irradiated by UV-A. Mass spectrometry analyses of HPLC fractionated extract led us to the identification of malvidin and cyanidin derivatives as the most active molecules able to counteract the negative effects induced by UV-A irradiation. Our results indicate, for the first time, that açai fruit is a valuable natural source for malvidin and cyanidin to be used as anti-stress molecules and represent good candidates for dietary intervention in the prevention of age related skin damage.</p>}},
  author       = {{Petruk, Ganna and Illiano, Anna and Del Giudice, Rita and Raiola, Assunta and Amoresano, Angela and Rigano, Maria Manuela and Piccoli, Renata and Monti, Daria Maria}},
  issn         = {{1011-1344}},
  keywords     = {{Antioxidants; Eukaryotic cells; Euterpe oleracea; Malvidin; Oxidative stress; Phenolics; UV-A radiations}},
  language     = {{eng}},
  month        = {{07}},
  pages        = {{42--51}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Photochemistry and Photobiology B: Biology}},
  title        = {{Malvidin and cyanidin derivatives from açai fruit (Euterpe oleracea Mart.) counteract UV-A-induced oxidative stress in immortalized fibroblasts}},
  url          = {{http://dx.doi.org/10.1016/j.jphotobiol.2017.05.013}},
  doi          = {{10.1016/j.jphotobiol.2017.05.013}},
  volume       = {{172}},
  year         = {{2017}},
}