Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone
(2019) In Talanta 191. p.202-208- Abstract
A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent... (More)
A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.
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- author
- Foubert, Astrid ; Beloglazova, Natalia V. ; Hedström, Martin LU and De Saeger, Sarah
- organization
- publishing date
- 2019-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Capacitive immunosensor, Mycotoxin, Polytyramine, Self-assembled monolayers, Zearalenone
- in
- Talanta
- volume
- 191
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85052501622
- pmid:30262050
- ISSN
- 0039-9140
- DOI
- 10.1016/j.talanta.2018.08.062
- language
- English
- LU publication?
- yes
- id
- bbd0c87e-4dae-45ec-b8a6-d00041457cba
- date added to LUP
- 2018-09-14 13:07:51
- date last changed
- 2024-07-23 22:19:05
@article{bbd0c87e-4dae-45ec-b8a6-d00041457cba, abstract = {{<p>A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL<sup>−1</sup>. It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.</p>}}, author = {{Foubert, Astrid and Beloglazova, Natalia V. and Hedström, Martin and De Saeger, Sarah}}, issn = {{0039-9140}}, keywords = {{Capacitive immunosensor; Mycotoxin; Polytyramine; Self-assembled monolayers; Zearalenone}}, language = {{eng}}, month = {{01}}, pages = {{202--208}}, publisher = {{Elsevier}}, series = {{Talanta}}, title = {{Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone}}, url = {{http://dx.doi.org/10.1016/j.talanta.2018.08.062}}, doi = {{10.1016/j.talanta.2018.08.062}}, volume = {{191}}, year = {{2019}}, }