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Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC

Dudziak, Diana ; Heger, Lukas ; Agace, William W. LU ; Bakker, Joyce ; de Gruijl, Tanja D. ; Dress, Regine J. ; Dutertre, Charles Antoine ; Fenton, Thomas M. ; Fransen, Marieke F. and Ginhoux, Florent , et al. (2025) In European Journal of Immunology 55(1).
Abstract

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including... (More)

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dendritic cells, flow cytometry, nonlymphoid tissues, tumor, tumor-draining lymph node
in
European Journal of Immunology
volume
55
issue
1
article number
2250325
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85211784866
  • pmid:39668411
ISSN
0014-2980
DOI
10.1002/eji.202250325
language
English
LU publication?
yes
id
bbf9155d-5c20-4ddb-8569-a17ea53e2c73
date added to LUP
2025-01-30 11:03:31
date last changed
2025-07-18 00:53:50
@article{bbf9155d-5c20-4ddb-8569-a17ea53e2c73,
  abstract     = {{<p>This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.</p>}},
  author       = {{Dudziak, Diana and Heger, Lukas and Agace, William W. and Bakker, Joyce and de Gruijl, Tanja D. and Dress, Regine J. and Dutertre, Charles Antoine and Fenton, Thomas M. and Fransen, Marieke F. and Ginhoux, Florent and Heyman, Oded and Horev, Yael and Hornsteiner, Florian and Kandiah, Vinitha and Kles, Paz and Lubin, Ruth and Mizraji, Gabriel and Prokopi, Anastasia and Saar, Or and Sopper, Sieghart and Stoitzner, Patrizia and Strandt, Helen and Sykora, Martina M. and Toffoli, Elisa C. and Tripp, Christoph H. and van Pul, Kim and van de Ven, Rieneke and Wilensky, Asaf and Yona, Simon and Zelle-Rieser, Claudia}},
  issn         = {{0014-2980}},
  keywords     = {{dendritic cells; flow cytometry; nonlymphoid tissues; tumor; tumor-draining lymph node}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{European Journal of Immunology}},
  title        = {{Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC}},
  url          = {{http://dx.doi.org/10.1002/eji.202250325}},
  doi          = {{10.1002/eji.202250325}},
  volume       = {{55}},
  year         = {{2025}},
}