Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Layer-by-layer technique as a new approach to produce nanostructured films containing phospholipids as transducers in sensing applications

Aoki, P. H.B. ; Volpati, D. LU ; Riul, A. ; Caetano, W. and Constantino, C. J.L. (2009) In Langmuir 25(4). p.2331-2338
Abstract

Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concentrations (nM level) of a phenothiazine compound were detected through impedance spectroscopy. Both negative l,2-dipalmitoyl-sn-3-glycero-[phosphor-rac-(l-glycerol)] (DPPG) and zwitterionic L-α-l,2-dipalmitoyl-sn-3-glycero-phosphatidylcholine (DPPC) phospholipids were used to produce the LbL films, whose molecular architecture was monitored... (More)

Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concentrations (nM level) of a phenothiazine compound were detected through impedance spectroscopy. Both negative l,2-dipalmitoyl-sn-3-glycero-[phosphor-rac-(l-glycerol)] (DPPG) and zwitterionic L-α-l,2-dipalmitoyl-sn-3-glycero-phosphatidylcholine (DPPC) phospholipids were used to produce the LbL films, whose molecular architecture was monitored combining spectroscopy and microscopy at micro and nanoscales. The sensor array was complemented by Langmuir - Blodgett (LB) monolayers of DPPG and DPPC deposited onto Pt interdigitated electrodes as well. It was found that the distinct molecular architecture presented by both LbL and LB films plays a key role on the sensitivity of the sensor array with the importance of the LbL films being demonstrated by principal component analysis (PCA).

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
25
issue
4
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:19161323
  • scopus:63249088790
ISSN
0743-7463
DOI
10.1021/la802696j
language
English
LU publication?
no
id
be3fd5f0-1aae-407c-86cb-b7ee265dc76b
date added to LUP
2019-05-17 14:38:52
date last changed
2024-01-01 05:16:18
@article{be3fd5f0-1aae-407c-86cb-b7ee265dc76b,
  abstract     = {{<p>Phospholipids are widely used as mimetic systems to exploit interactions involving biological membranes and pharmacological drugs. In this work, the layer-by-layer (LbL) technique was used as a new approach to produce multilayered thin films containing biological phospholipids applied as transducers onto Pt interdigitated electrodes forming sensing units of an electronic tongue system. Low concentrations (nM level) of a phenothiazine compound were detected through impedance spectroscopy. Both negative l,2-dipalmitoyl-sn-3-glycero-[phosphor-rac-(l-glycerol)] (DPPG) and zwitterionic L-α-l,2-dipalmitoyl-sn-3-glycero-phosphatidylcholine (DPPC) phospholipids were used to produce the LbL films, whose molecular architecture was monitored combining spectroscopy and microscopy at micro and nanoscales. The sensor array was complemented by Langmuir - Blodgett (LB) monolayers of DPPG and DPPC deposited onto Pt interdigitated electrodes as well. It was found that the distinct molecular architecture presented by both LbL and LB films plays a key role on the sensitivity of the sensor array with the importance of the LbL films being demonstrated by principal component analysis (PCA).</p>}},
  author       = {{Aoki, P. H.B. and Volpati, D. and Riul, A. and Caetano, W. and Constantino, C. J.L.}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{4}},
  pages        = {{2331--2338}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{Layer-by-layer technique as a new approach to produce nanostructured films containing phospholipids as transducers in sensing applications}},
  url          = {{http://dx.doi.org/10.1021/la802696j}},
  doi          = {{10.1021/la802696j}},
  volume       = {{25}},
  year         = {{2009}},
}