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‘Hot Spots’ of creatine kinase localization in brain : Cerebellum, hippocampus and choroid plexus

Kaldis, Philipp LU orcid ; Hemmer, Wolfram ; Zanolla, Else ; Holtzman, David and Wallimann, Theo (1996) In Developmental Neuroscience 18(5-6). p.542-554
Abstract

Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence of a Mib-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mib-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these... (More)

Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence of a Mib-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mib-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mia-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mia-CK, however, was also very prominent in Bergmann glial cells of the molecular layer. Thus, an isoenzyme-specific differential localization of the two mitochondrial Mi-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mia-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK. The possible implications of a CK/PCr circuit at these various cellular locations of the brain are discussed with respect to normal brain physiology and pathology.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Bergmann glia, Brain energy metabolism, Cerebellum, Creatine kinase, Epithelial cells of choroid plexus, Granular and pyramidal cells of hippocampus, Immunofluorescence microscopy, Purkinje neurons
in
Developmental Neuroscience
volume
18
issue
5-6
pages
542 - 554
publisher
Karger
external identifiers
  • pmid:8940630
  • scopus:0029842655
ISSN
0378-5866
DOI
10.1159/000111452
language
English
LU publication?
no
id
bf531fd3-b519-46d3-ab33-253e34b0fac2
date added to LUP
2019-09-18 14:37:34
date last changed
2024-06-12 01:55:59
@article{bf531fd3-b519-46d3-ab33-253e34b0fac2,
  abstract     = {{<p>Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mi<sub>b</sub>-CK) and revealed the presence of a Mi<sub>b</sub>-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mi<sub>b</sub>-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca<sup>2+</sup> levels (Ca<sup>2+</sup> spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mi<sub>a</sub>-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mi<sub>a</sub>-CK, however, was also very prominent in Bergmann glial cells of the molecular layer. Thus, an isoenzyme-specific differential localization of the two mitochondrial Mi-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mi<sub>a</sub>-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK. The possible implications of a CK/PCr circuit at these various cellular locations of the brain are discussed with respect to normal brain physiology and pathology.</p>}},
  author       = {{Kaldis, Philipp and Hemmer, Wolfram and Zanolla, Else and Holtzman, David and Wallimann, Theo}},
  issn         = {{0378-5866}},
  keywords     = {{Bergmann glia; Brain energy metabolism; Cerebellum; Creatine kinase; Epithelial cells of choroid plexus; Granular and pyramidal cells of hippocampus; Immunofluorescence microscopy; Purkinje neurons}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{5-6}},
  pages        = {{542--554}},
  publisher    = {{Karger}},
  series       = {{Developmental Neuroscience}},
  title        = {{‘Hot Spots’ of creatine kinase localization in brain : Cerebellum, hippocampus and choroid plexus}},
  url          = {{http://dx.doi.org/10.1159/000111452}},
  doi          = {{10.1159/000111452}},
  volume       = {{18}},
  year         = {{1996}},
}