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Fast determination of whole blood glucose with a calorimetric micro-biosensor

Xie, Bin LU ; Hedberg, Ulrika ; Mecklenburg, Michael and Danielsson, Bengt LU (1993) In Sensors and Actuators B: Chemical 15(1-3). p.141-144
Abstract

Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Sensors and Actuators B: Chemical
volume
15
issue
1-3
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:0027647443
ISSN
0925-4005
DOI
10.1016/0925-4005(93)85040-H
language
English
LU publication?
yes
id
c0528832-89ba-4226-ab91-cfb1ef6c639b
date added to LUP
2021-10-27 16:05:33
date last changed
2021-11-01 11:50:44
@article{c0528832-89ba-4226-ab91-cfb1ef6c639b,
  abstract     = {{<p>Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.</p>}},
  author       = {{Xie, Bin and Hedberg, Ulrika and Mecklenburg, Michael and Danielsson, Bengt}},
  issn         = {{0925-4005}},
  language     = {{eng}},
  number       = {{1-3}},
  pages        = {{141--144}},
  publisher    = {{Elsevier}},
  series       = {{Sensors and Actuators B: Chemical}},
  title        = {{Fast determination of whole blood glucose with a calorimetric micro-biosensor}},
  url          = {{http://dx.doi.org/10.1016/0925-4005(93)85040-H}},
  doi          = {{10.1016/0925-4005(93)85040-H}},
  volume       = {{15}},
  year         = {{1993}},
}