The predominant protein in human seminal coagulate
(1985) In Scandinavian Journal of Clinical and Laboratory Investigation 45(7). p.635-641- Abstract
The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction... (More)
The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction of ejaculated semen to a series of labile proteins. These proteins are further cleaved to peptides of successively decreasing size after completed liquefaction. The cleavage of the HMW-SV-protein is the major cause of the fast shift of the electrophoretic pattern of seminal proteins if semen is stored without protease inhibitors.
(Less)
- author
- Lilja, H. LU and Laurell, C. B. LU
- organization
- publishing date
- 1985
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Inhibin, Prostate, Protease inhibitors, Proteolytic enzymes, Semen
- in
- Scandinavian Journal of Clinical and Laboratory Investigation
- volume
- 45
- issue
- 7
- pages
- 635 - 641
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:2866578
- scopus:0022239505
- ISSN
- 0036-5513
- DOI
- 10.3109/00365518509155271
- language
- English
- LU publication?
- yes
- id
- c138c476-a2a7-428c-b8ea-f85e564d05cc
- date added to LUP
- 2022-12-06 16:53:07
- date last changed
- 2024-01-18 15:55:46
@article{c138c476-a2a7-428c-b8ea-f85e564d05cc, abstract = {{<p>The predominant protein in human seminal vesicle secretion constitutes the structural protein of coagulated semen. This high molecular weight protein (HMW-SV-protein) is stable in seminal vesicle secretion during in vitro storage at 37 d̀C for at least 20 h, but is rapidly cleaved on mixing with prostatic proteases. Seminal coagulate, washed free of souble components, is dissoluble by 2 to 3 mol/1 of guanidine-HCl. Although dithiothreitol added to seminal coagulate does not liquify the clot, complexes between HMW-SV-proteins are broken up by reduction under denaturing conditions, which suggests that the non-covalent linkages of HMW-SV-proteins are essential in the clot. Prostatic proteases cleave the HMW-SV-protein during liquefaction of ejaculated semen to a series of labile proteins. These proteins are further cleaved to peptides of successively decreasing size after completed liquefaction. The cleavage of the HMW-SV-protein is the major cause of the fast shift of the electrophoretic pattern of seminal proteins if semen is stored without protease inhibitors.</p>}}, author = {{Lilja, H. and Laurell, C. B.}}, issn = {{0036-5513}}, keywords = {{Inhibin; Prostate; Protease inhibitors; Proteolytic enzymes; Semen}}, language = {{eng}}, number = {{7}}, pages = {{635--641}}, publisher = {{Informa Healthcare}}, series = {{Scandinavian Journal of Clinical and Laboratory Investigation}}, title = {{The predominant protein in human seminal coagulate}}, url = {{http://dx.doi.org/10.3109/00365518509155271}}, doi = {{10.3109/00365518509155271}}, volume = {{45}}, year = {{1985}}, }