Characterization of histamine receptors mediating contraction and relaxation of the ovarian follicle wall
(1987) In International Journal of Fertility 32(5). p.399-406- Abstract
Histamine is known to be present in ovarian tissue and may play a role in the ovulation process. The effect of histamine on the follicular smooth musculature was investigated using strips from the protruding part of mature bovine follicles, mounted in vitro for registration of isometric motor activity. Histamine contracted the preparation in a concentration-dependent manner. The response was inhibited competitively by the specific H1 receptor antagonist pyrilamine while the adrenergic α-receptor antagonist phentolamine had no clear-cut effect. A Schild plot revealed a pA2 value of 8.81, corresponding to a mean K(B) value (dissociation constant for receptor-antagonist complex) of (7.5 ± 3.2) x 10-9 M.... (More)
Histamine is known to be present in ovarian tissue and may play a role in the ovulation process. The effect of histamine on the follicular smooth musculature was investigated using strips from the protruding part of mature bovine follicles, mounted in vitro for registration of isometric motor activity. Histamine contracted the preparation in a concentration-dependent manner. The response was inhibited competitively by the specific H1 receptor antagonist pyrilamine while the adrenergic α-receptor antagonist phentolamine had no clear-cut effect. A Schild plot revealed a pA2 value of 8.81, corresponding to a mean K(B) value (dissociation constant for receptor-antagonist complex) of (7.5 ± 3.2) x 10-9 M. After potassium depolarization and blockade of the contractile H1-receptors with pyrilamine, histamine induced a concentration-dependent relaxation of the follicle wall preparation. This response could be inhibited by the H2-receptor antagonist cimetidine (which also potentiated the contractile effect in the absence of pyrilamine). The pA2 value for the cimetidine-induced inhibition was 6.25, and K(B) was found to be (6.5 ± 3.0) x 10-7 M. The β-receptor antagonist propranolol was effective only in very high concentrations. It is suggested that a possible role for histamine during follicle rupture is mediated via specific receptors in the follicular smooth musculature.
(Less)
- author
- Schmidt, G. ; Kannisto, P. LU ; Owman, Ch and Walles, B.
- organization
- publishing date
- 1987-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Fertility
- volume
- 32
- issue
- 5
- pages
- 399 - 406
- publisher
- MSP International
- external identifiers
-
- pmid:2889689
- scopus:0023618230
- ISSN
- 0020-725X
- language
- English
- LU publication?
- yes
- id
- c13eb07a-c72d-437a-8cd5-61e9b5d7628e
- date added to LUP
- 2020-01-20 15:36:32
- date last changed
- 2024-01-02 04:34:18
@article{c13eb07a-c72d-437a-8cd5-61e9b5d7628e,
abstract = {{<p>Histamine is known to be present in ovarian tissue and may play a role in the ovulation process. The effect of histamine on the follicular smooth musculature was investigated using strips from the protruding part of mature bovine follicles, mounted in vitro for registration of isometric motor activity. Histamine contracted the preparation in a concentration-dependent manner. The response was inhibited competitively by the specific H<sub>1</sub> receptor antagonist pyrilamine while the adrenergic α-receptor antagonist phentolamine had no clear-cut effect. A Schild plot revealed a pA<sub>2</sub> value of 8.81, corresponding to a mean K(B) value (dissociation constant for receptor-antagonist complex) of (7.5 ± 3.2) x 10<sup>-9</sup> M. After potassium depolarization and blockade of the contractile H<sub>1</sub>-receptors with pyrilamine, histamine induced a concentration-dependent relaxation of the follicle wall preparation. This response could be inhibited by the H<sub>2</sub>-receptor antagonist cimetidine (which also potentiated the contractile effect in the absence of pyrilamine). The pA<sub>2</sub> value for the cimetidine-induced inhibition was 6.25, and K(B) was found to be (6.5 ± 3.0) x 10<sup>-7</sup> M. The β-receptor antagonist propranolol was effective only in very high concentrations. It is suggested that a possible role for histamine during follicle rupture is mediated via specific receptors in the follicular smooth musculature.</p>}},
author = {{Schmidt, G. and Kannisto, P. and Owman, Ch and Walles, B.}},
issn = {{0020-725X}},
language = {{eng}},
month = {{12}},
number = {{5}},
pages = {{399--406}},
publisher = {{MSP International}},
series = {{International Journal of Fertility}},
title = {{Characterization of histamine receptors mediating contraction and relaxation of the ovarian follicle wall}},
volume = {{32}},
year = {{1987}},
}