Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population
(2017) In International Journal of Laboratory Hematology 39. p.76-85- Abstract
We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow™ LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone®) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these... (More)
We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow™ LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone®) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these cases, a final diagnosis could be rendered in 106 cases (52%), and in the remainder, additional B-cell immunophenotyping was performed. In 20 (3%) samples, an aberrant T-cell population was confirmed by additional testing. Of 425 samples diagnosed as normal/reactive lymphoid tissue, 50 (12%) required additional immunophenotyping, mostly due to an abnormal CD4/CD8 ratio. The LPD-ST tube significantly minimizes the need for additional testing, improves the turn-around time, and reduces the cost of LPD immunophenotyping. It is also suitable for investigating paucicellular samples such as cerebrospinal fluid or fine needle aspirates.
(Less)
- author
- Rajab, Amr ; Axler, Olof LU ; Leung, Jessie ; Wozniak, Malgorzata and Porwit, Anna LU
- organization
- publishing date
- 2017-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lymphocyte, lymphoma, multiparameter flow cytometry, screening
- in
- International Journal of Laboratory Hematology
- volume
- 39
- pages
- 10 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:28447425
- wos:000400189400013
- scopus:85018677412
- ISSN
- 1751-5521
- DOI
- 10.1111/ijlh.12678
- language
- English
- LU publication?
- yes
- id
- c46f6624-e00b-4d5a-bdc8-103248bfbb33
- date added to LUP
- 2017-06-08 14:49:45
- date last changed
- 2024-07-07 19:23:24
@article{c46f6624-e00b-4d5a-bdc8-103248bfbb33,
abstract = {{<p>We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow<sup>™</sup> LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone<sup>®</sup>) on 649 patient samples in Sweden. In 204 of 649 samples (31%), a monotypic B-cell population was found. Of these cases, a final diagnosis could be rendered in 106 cases (52%), and in the remainder, additional B-cell immunophenotyping was performed. In 20 (3%) samples, an aberrant T-cell population was confirmed by additional testing. Of 425 samples diagnosed as normal/reactive lymphoid tissue, 50 (12%) required additional immunophenotyping, mostly due to an abnormal CD4/CD8 ratio. The LPD-ST tube significantly minimizes the need for additional testing, improves the turn-around time, and reduces the cost of LPD immunophenotyping. It is also suitable for investigating paucicellular samples such as cerebrospinal fluid or fine needle aspirates.</p>}},
author = {{Rajab, Amr and Axler, Olof and Leung, Jessie and Wozniak, Malgorzata and Porwit, Anna}},
issn = {{1751-5521}},
keywords = {{lymphocyte; lymphoma; multiparameter flow cytometry; screening}},
language = {{eng}},
month = {{05}},
pages = {{76--85}},
publisher = {{Wiley-Blackwell}},
series = {{International Journal of Laboratory Hematology}},
title = {{Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population}},
url = {{http://dx.doi.org/10.1111/ijlh.12678}},
doi = {{10.1111/ijlh.12678}},
volume = {{39}},
year = {{2017}},
}