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The efficacy of silver-embedded polypropylene-grafted polyethylene glycol-coated ventricular catheters on prevention of shunt catheter infection in rats

Hazer, Derya Burcu LU orcid ; Mut, Melike ; Dinçer, Nazmiye ; Saribas, Zeynep ; Hazer, Baki and Özgen, Tunçalp (2012) In Child's Nervous System 28(6). p.839-846
Abstract

Purpose: Catheter-related infection is a major complication of ventriculoperitoneal shunt in children. The aim of this study is to determine inflammatory response and the efficacy of polypropylene-grafted polyethylene glycol (PP-g- PEG) copolymer and silver nanoparticle-embedded PP-g- PEG (Ag-PP-g-PEG) polymer-coated ventricular catheters on the prevention of catheter-related infections on a new experimental model of ventriculoperitoneal shunt in rats. Methods: Thirty six Wistar albino rats were divided into six groups: group 1, unprocessed sterile silicone catheterembedded group; group 2, sterile PP-g-PEG-coated catheter group; group 3, sterile Ag-PP-g-PEG-coated catheter group; group 4, infected unprocessed catheter group; group 5,... (More)

Purpose: Catheter-related infection is a major complication of ventriculoperitoneal shunt in children. The aim of this study is to determine inflammatory response and the efficacy of polypropylene-grafted polyethylene glycol (PP-g- PEG) copolymer and silver nanoparticle-embedded PP-g- PEG (Ag-PP-g-PEG) polymer-coated ventricular catheters on the prevention of catheter-related infections on a new experimental model of ventriculoperitoneal shunt in rats. Methods: Thirty six Wistar albino rats were divided into six groups: group 1, unprocessed sterile silicone catheterembedded group; group 2, sterile PP-g-PEG-coated catheter group; group 3, sterile Ag-PP-g-PEG-coated catheter group; group 4, infected unprocessed catheter group; group 5, infected PP-g-PEG-coated catheter group; and group 6, infected Ag-PP-g-PEG-coated catheter group, respectively. In all groups, 1-cm piece of designated catheters were placed into the cisterna magna. In groups 4, 5, and 6, all rats were infected with 0.2 mL of 10×106 colony forming units (CFU)/mL Staphylococcus epidermidis colonies before the catheters were placed. Thirty days after implantation, bacterial colonization in cerebrospinal fluid and on catheter pieces with inflammatory reaction in the brain parenchyma was analyzed quantitatively. Results: Sterile and infected Ag-PP-g-PEG-covered groups revealed significantly lower bacteria colony count on the catheter surface (ANOVA, 0±0, p<0.001; 1.08±0.18, p< 0.05, respectively). There was moderate inflammatory response in the parenchyma in group 4, but in groups 5 and 6, it was similar to that of the sterile group (ANOVA, 16.33± 3.02, p<0.001; 4.00±0.68, p<0.001, respectively). Conclusions: The PP-g-PEG, especially Ag-PP-g-PEG polymer-coated ventricular catheters are more effective in preventing the catheter-related infection and created the least inflammatory reaction in the periventricular parenchyma.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Polyethylene glycol, Polypropylene, Shunt catheter, Ventriculitis
in
Child's Nervous System
volume
28
issue
6
pages
839 - 846
publisher
Springer
external identifiers
  • scopus:84863715962
  • pmid:22373811
ISSN
0256-7040
DOI
10.1007/s00381-012-1729-5
language
English
LU publication?
no
id
c47cb8b5-0a87-40b4-ae7d-32b4cb81f220
date added to LUP
2025-05-11 22:36:02
date last changed
2025-06-09 01:29:56
@article{c47cb8b5-0a87-40b4-ae7d-32b4cb81f220,
  abstract     = {{<p>Purpose: Catheter-related infection is a major complication of ventriculoperitoneal shunt in children. The aim of this study is to determine inflammatory response and the efficacy of polypropylene-grafted polyethylene glycol (PP-g- PEG) copolymer and silver nanoparticle-embedded PP-g- PEG (Ag-PP-g-PEG) polymer-coated ventricular catheters on the prevention of catheter-related infections on a new experimental model of ventriculoperitoneal shunt in rats. Methods: Thirty six Wistar albino rats were divided into six groups: group 1, unprocessed sterile silicone catheterembedded group; group 2, sterile PP-g-PEG-coated catheter group; group 3, sterile Ag-PP-g-PEG-coated catheter group; group 4, infected unprocessed catheter group; group 5, infected PP-g-PEG-coated catheter group; and group 6, infected Ag-PP-g-PEG-coated catheter group, respectively. In all groups, 1-cm piece of designated catheters were placed into the cisterna magna. In groups 4, 5, and 6, all rats were infected with 0.2 mL of 10×10<sup>6</sup> colony forming units (CFU)/mL Staphylococcus epidermidis colonies before the catheters were placed. Thirty days after implantation, bacterial colonization in cerebrospinal fluid and on catheter pieces with inflammatory reaction in the brain parenchyma was analyzed quantitatively. Results: Sterile and infected Ag-PP-g-PEG-covered groups revealed significantly lower bacteria colony count on the catheter surface (ANOVA, 0±0, p&lt;0.001; 1.08±0.18, p&lt; 0.05, respectively). There was moderate inflammatory response in the parenchyma in group 4, but in groups 5 and 6, it was similar to that of the sterile group (ANOVA, 16.33± 3.02, p&lt;0.001; 4.00±0.68, p&lt;0.001, respectively). Conclusions: The PP-g-PEG, especially Ag-PP-g-PEG polymer-coated ventricular catheters are more effective in preventing the catheter-related infection and created the least inflammatory reaction in the periventricular parenchyma.</p>}},
  author       = {{Hazer, Derya Burcu and Mut, Melike and Dinçer, Nazmiye and Saribas, Zeynep and Hazer, Baki and Özgen, Tunçalp}},
  issn         = {{0256-7040}},
  keywords     = {{Polyethylene glycol; Polypropylene; Shunt catheter; Ventriculitis}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{839--846}},
  publisher    = {{Springer}},
  series       = {{Child's Nervous System}},
  title        = {{The efficacy of silver-embedded polypropylene-grafted polyethylene glycol-coated ventricular catheters on prevention of shunt catheter infection in rats}},
  url          = {{http://dx.doi.org/10.1007/s00381-012-1729-5}},
  doi          = {{10.1007/s00381-012-1729-5}},
  volume       = {{28}},
  year         = {{2012}},
}