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Modified Nucleic Acid Extraction Protocol for Humic-Rich Permafrost Peat Samples v1

Li, Yueh-Fen ; Mondav, Rhiannon LU orcid and Rich, Virginia (2024)
Abstract
Slight modifications to the manufacturer’s protocol (Qiagen #12966-10, now discontinued and replaced with #12988-10) enabled the successful extraction of high-quality DNA and RNA from permafrost peat samples. Integrating the DNA and RNA purification steps immediately after the extraction process helped streamline the workflow, resulting in consistent quality nucleic acids suitable for downstream sequencing and analysis. This improved approach optimizes the extraction process, ensuring more reliable and efficient results for permafrost peat studies.
Please use this url to cite or link to this publication:
author
; and
author collaboration
organization
publishing date
type
Working paper/Preprint
publication status
published
subject
keywords
DNA/*analysis/isolation & purification, RNA analysis, peat
publisher
Springer Nature
DOI
10.17504/protocols.io.yxmvm244bg3p/v1
language
English
LU publication?
yes
id
c4b3113b-d2cd-410c-b11b-4c6a8461c83d
alternative location
https://www.protocols.io/view/modified-nucleic-acid-extraction-protocol-for-humi-csvywe7w
date added to LUP
2024-12-11 11:23:01
date last changed
2025-04-04 14:13:23
@misc{c4b3113b-d2cd-410c-b11b-4c6a8461c83d,
  abstract     = {{Slight modifications to the manufacturer’s protocol (Qiagen #12966-10, now discontinued and replaced with #12988-10) enabled the successful extraction of high-quality DNA and RNA from permafrost peat samples. Integrating the DNA and RNA purification steps immediately after the extraction process helped streamline the workflow, resulting in consistent quality nucleic acids suitable for downstream sequencing and analysis. This improved approach optimizes the extraction process, ensuring more reliable and efficient results for permafrost peat studies.}},
  author       = {{Li, Yueh-Fen and Mondav, Rhiannon and Rich, Virginia}},
  keywords     = {{DNA/*analysis/isolation & purification; RNA analysis; peat}},
  language     = {{eng}},
  month        = {{11}},
  note         = {{Preprint}},
  publisher    = {{Springer Nature}},
  title        = {{Modified Nucleic Acid Extraction Protocol for Humic-Rich Permafrost Peat Samples v1}},
  url          = {{http://dx.doi.org/10.17504/protocols.io.yxmvm244bg3p/v1}},
  doi          = {{10.17504/protocols.io.yxmvm244bg3p/v1}},
  year         = {{2024}},
}