Highly efficient acoustophoretic single cell-supernatant separation inside nanoliter droplets
(2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.260-261- Abstract
This contribution reports a novel method for cell-supernatant separation inside nanoliter droplets with separation efficiencies for single cells of 100% at flow speeds of 2 mm/s. Cells are focused in nanoliter droplets using bulk acoustic waves before the droplet is split at a bifurcation into a supernatant- and a cell-containing droplet. The method enables upconcentration of cells, cell washing, and the separation of supernatant.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c8dc7938-23a8-4565-9c6f-3efe28ad897c
- author
- Gerlt, Michael
LU
; Haidas, Dominik ; Ratschat, Alexandre ; Suter, Philipp ; Dittrich, Petra and Dual, Jürg
- publishing date
- 2020
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Cell-supernatant separation, Intra droplet manipulation, Single-cell
- host publication
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- series title
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
- conference location
- Virtual, Online
- conference dates
- 2020-10-04 - 2020-10-09
- external identifiers
-
- scopus:85098292067
- ISBN
- 9781733419017
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2020 CBMS-0001
- id
- c8dc7938-23a8-4565-9c6f-3efe28ad897c
- date added to LUP
- 2024-07-01 14:18:07
- date last changed
- 2024-07-11 14:51:31
@inproceedings{c8dc7938-23a8-4565-9c6f-3efe28ad897c, abstract = {{<p>This contribution reports a novel method for cell-supernatant separation inside nanoliter droplets with separation efficiencies for single cells of 100% at flow speeds of 2 mm/s. Cells are focused in nanoliter droplets using bulk acoustic waves before the droplet is split at a bifurcation into a supernatant- and a cell-containing droplet. The method enables upconcentration of cells, cell washing, and the separation of supernatant.</p>}}, author = {{Gerlt, Michael and Haidas, Dominik and Ratschat, Alexandre and Suter, Philipp and Dittrich, Petra and Dual, Jürg}}, booktitle = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419017}}, keywords = {{Cell-supernatant separation; Intra droplet manipulation; Single-cell}}, language = {{eng}}, pages = {{260--261}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, title = {{Highly efficient acoustophoretic single cell-supernatant separation inside nanoliter droplets}}, year = {{2020}}, }