Clonal evolution through genetic bottlenecks and telomere attrition : Potential threats to in vitro data reproducibility
(2019) In Genes Chromosomes and Cancer 58(7). p.452-461- Abstract
Tissue cultures of immortalized human cells, also known as established cell lines, are broadly accessible and cost-efficient tools for biomedical research. We here review potential genetic sources of systematic error in cell line experiments due to clonal evolution in vitro. In particular, the authors highlight alterations in telomere function over prolonged culture and population bottlenecks, respectively, as two commonly overlooked phenomena that can result in significant alterations in cell line genotypes over just one or a few passages in vitro. These alterations may include changes in mutation status of oncogenes and large scale chromosomal imbalances. We introduce a simple list of factors to be avoided in order to reduce the risk... (More)
Tissue cultures of immortalized human cells, also known as established cell lines, are broadly accessible and cost-efficient tools for biomedical research. We here review potential genetic sources of systematic error in cell line experiments due to clonal evolution in vitro. In particular, the authors highlight alterations in telomere function over prolonged culture and population bottlenecks, respectively, as two commonly overlooked phenomena that can result in significant alterations in cell line genotypes over just one or a few passages in vitro. These alterations may include changes in mutation status of oncogenes and large scale chromosomal imbalances. We introduce a simple list of factors to be avoided in order to reduce the risk of data misinterpretation due to clonal evolution, including unacknowledged in vitro selection pressures, prolonged culture per se, harsh population size reductions, experiments at early phases after establishment, and the employment of cell lines not sufficiently analyzed by high resolution genetic techniques.
(Less)
- author
- Gisselsson, David LU ; Lichtenzstejn, Daniel ; Kachko, Polina ; Karlsson, Jenny LU ; Manor, Esther and Mai, Sabine
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cancer, cell lines, clonal evolution, genetic bottleneck, telomeres
- in
- Genes Chromosomes and Cancer
- volume
- 58
- issue
- 7
- pages
- 452 - 461
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85057561302
- pmid:30255964
- ISSN
- 1045-2257
- DOI
- 10.1002/gcc.22685
- language
- English
- LU publication?
- yes
- id
- cc3f23bb-77f0-4bff-b0d4-002333776d07
- date added to LUP
- 2018-12-20 14:10:57
- date last changed
- 2024-06-12 04:16:22
@article{cc3f23bb-77f0-4bff-b0d4-002333776d07,
abstract = {{<p>Tissue cultures of immortalized human cells, also known as established cell lines, are broadly accessible and cost-efficient tools for biomedical research. We here review potential genetic sources of systematic error in cell line experiments due to clonal evolution in vitro. In particular, the authors highlight alterations in telomere function over prolonged culture and population bottlenecks, respectively, as two commonly overlooked phenomena that can result in significant alterations in cell line genotypes over just one or a few passages in vitro. These alterations may include changes in mutation status of oncogenes and large scale chromosomal imbalances. We introduce a simple list of factors to be avoided in order to reduce the risk of data misinterpretation due to clonal evolution, including unacknowledged in vitro selection pressures, prolonged culture per se, harsh population size reductions, experiments at early phases after establishment, and the employment of cell lines not sufficiently analyzed by high resolution genetic techniques.</p>}},
author = {{Gisselsson, David and Lichtenzstejn, Daniel and Kachko, Polina and Karlsson, Jenny and Manor, Esther and Mai, Sabine}},
issn = {{1045-2257}},
keywords = {{cancer; cell lines; clonal evolution; genetic bottleneck; telomeres}},
language = {{eng}},
number = {{7}},
pages = {{452--461}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Genes Chromosomes and Cancer}},
title = {{Clonal evolution through genetic bottlenecks and telomere attrition : Potential threats to in vitro data reproducibility}},
url = {{http://dx.doi.org/10.1002/gcc.22685}},
doi = {{10.1002/gcc.22685}},
volume = {{58}},
year = {{2019}},
}