Intraspecific nucleotide variation at the pheromone binding protein locus in the turnip moth, Agrotis segetum
(1999) In Insect Molecular Biology 8(4). p.481-490- Abstract
Inter- and intraspecific amino acid variability in the pheromone binding proteins (PBPs) of the Lepidoptera is believed to contribute to a molecular mechanism of pheromone blend discrimination. Messenger RNA coding for PBP sequence in Agrotis segetum (Noctuidae) was cloned, and nucleotide and inferred amino acid variation across a 769-bp region of a PBP locus was studied in two populations. A single gene copy was fully sequenced, revealing an intron/exon structure conserved with distant saturniids. While several nucleotide substitutions are predicted to result in amino acid replacement, tests for the presence of natural selection suggest that the observed variation is neutral. A phylogenetic analysis provides evidence that the two... (More)
Inter- and intraspecific amino acid variability in the pheromone binding proteins (PBPs) of the Lepidoptera is believed to contribute to a molecular mechanism of pheromone blend discrimination. Messenger RNA coding for PBP sequence in Agrotis segetum (Noctuidae) was cloned, and nucleotide and inferred amino acid variation across a 769-bp region of a PBP locus was studied in two populations. A single gene copy was fully sequenced, revealing an intron/exon structure conserved with distant saturniids. While several nucleotide substitutions are predicted to result in amino acid replacement, tests for the presence of natural selection suggest that the observed variation is neutral. A phylogenetic analysis provides evidence that the two populations are in the process of genetic isolation.
(Less)
- author
- LaForest, Siana M. ; Prestwich, G. D. and Löfstedt, C. LU
- organization
- publishing date
- 1999-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Agrotis segetum, Odourant binding proteins, Pheromone binding proteins
- in
- Insect Molecular Biology
- volume
- 8
- issue
- 4
- pages
- 10 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0033230538
- pmid:10620043
- ISSN
- 0962-1075
- DOI
- 10.1046/j.1365-2583.1999.00143.x
- project
- Evolutionary mechanisms of pheromone divergence in Lepidoptera
- language
- English
- LU publication?
- yes
- id
- d1075432-4619-45bc-88a5-5b52988679d5
- date added to LUP
- 2020-05-26 15:22:45
- date last changed
- 2024-06-26 17:08:50
@article{d1075432-4619-45bc-88a5-5b52988679d5,
abstract = {{<p>Inter- and intraspecific amino acid variability in the pheromone binding proteins (PBPs) of the Lepidoptera is believed to contribute to a molecular mechanism of pheromone blend discrimination. Messenger RNA coding for PBP sequence in Agrotis segetum (Noctuidae) was cloned, and nucleotide and inferred amino acid variation across a 769-bp region of a PBP locus was studied in two populations. A single gene copy was fully sequenced, revealing an intron/exon structure conserved with distant saturniids. While several nucleotide substitutions are predicted to result in amino acid replacement, tests for the presence of natural selection suggest that the observed variation is neutral. A phylogenetic analysis provides evidence that the two populations are in the process of genetic isolation.</p>}},
author = {{LaForest, Siana M. and Prestwich, G. D. and Löfstedt, C.}},
issn = {{0962-1075}},
keywords = {{Agrotis segetum; Odourant binding proteins; Pheromone binding proteins}},
language = {{eng}},
month = {{11}},
number = {{4}},
pages = {{481--490}},
publisher = {{Wiley-Blackwell}},
series = {{Insect Molecular Biology}},
title = {{Intraspecific nucleotide variation at the pheromone binding protein locus in the turnip moth, Agrotis segetum}},
url = {{http://dx.doi.org/10.1046/j.1365-2583.1999.00143.x}},
doi = {{10.1046/j.1365-2583.1999.00143.x}},
volume = {{8}},
year = {{1999}},
}