A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans
(2024) In Nature Communications 15. p.1-14- Abstract
Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane... (More)
Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.
(Less)
- author
- Cao, Wei
; Fan, Qi
; Amparado, Gemmarie
; Begic, Dean
; Godini, Rasoul
; Gopal, Sandeep
LU
and Pocock, Roger
- organization
- publishing date
- 2024-08-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Caenorhabditis elegans/genetics, Germ Cells/metabolism, Caenorhabditis elegans Proteins/metabolism, Gene Expression Regulation, Developmental, Cell Differentiation/genetics, RNA-Binding Proteins/metabolism, Fertility/genetics, DNA-Binding Proteins/metabolism, Cell Proliferation/genetics, Cell Cycle/genetics, Male, Gene Regulatory Networks
- in
- Nature Communications
- volume
- 15
- article number
- 6884
- pages
- 1 - 14
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:39128930
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-024-51212-0
- language
- English
- LU publication?
- yes
- additional info
- © 2024. The Author(s).
- id
- d1fd73c5-6350-4832-afd4-969bf31a88c5
- date added to LUP
- 2024-08-18 08:12:08
- date last changed
- 2024-08-19 07:42:14
@article{d1fd73c5-6350-4832-afd4-969bf31a88c5, abstract = {{<p>Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.</p>}}, author = {{Cao, Wei and Fan, Qi and Amparado, Gemmarie and Begic, Dean and Godini, Rasoul and Gopal, Sandeep and Pocock, Roger}}, issn = {{2041-1723}}, keywords = {{Animals; Caenorhabditis elegans/genetics; Germ Cells/metabolism; Caenorhabditis elegans Proteins/metabolism; Gene Expression Regulation, Developmental; Cell Differentiation/genetics; RNA-Binding Proteins/metabolism; Fertility/genetics; DNA-Binding Proteins/metabolism; Cell Proliferation/genetics; Cell Cycle/genetics; Male; Gene Regulatory Networks}}, language = {{eng}}, month = {{08}}, pages = {{1--14}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans}}, url = {{http://dx.doi.org/10.1038/s41467-024-51212-0}}, doi = {{10.1038/s41467-024-51212-0}}, volume = {{15}}, year = {{2024}}, }