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A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans

Cao, Wei ; Fan, Qi ; Amparado, Gemmarie ; Begic, Dean ; Godini, Rasoul ; Gopal, Sandeep LU orcid and Pocock, Roger (2024) In Nature Communications 15. p.1-14
Abstract

Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane... (More)

Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.

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organization
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Contribution to journal
publication status
published
subject
keywords
Animals, Caenorhabditis elegans/genetics, Germ Cells/metabolism, Caenorhabditis elegans Proteins/metabolism, Gene Expression Regulation, Developmental, Cell Differentiation/genetics, RNA-Binding Proteins/metabolism, Fertility/genetics, DNA-Binding Proteins/metabolism, Cell Proliferation/genetics, Cell Cycle/genetics, Male, Gene Regulatory Networks
in
Nature Communications
volume
15
article number
6884
pages
1 - 14
publisher
Nature Publishing Group
external identifiers
  • pmid:39128930
ISSN
2041-1723
DOI
10.1038/s41467-024-51212-0
language
English
LU publication?
yes
additional info
© 2024. The Author(s).
id
d1fd73c5-6350-4832-afd4-969bf31a88c5
date added to LUP
2024-08-18 08:12:08
date last changed
2024-08-19 07:42:14
@article{d1fd73c5-6350-4832-afd4-969bf31a88c5,
  abstract     = {{<p>Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.</p>}},
  author       = {{Cao, Wei and Fan, Qi and Amparado, Gemmarie and Begic, Dean and Godini, Rasoul and Gopal, Sandeep and Pocock, Roger}},
  issn         = {{2041-1723}},
  keywords     = {{Animals; Caenorhabditis elegans/genetics; Germ Cells/metabolism; Caenorhabditis elegans Proteins/metabolism; Gene Expression Regulation, Developmental; Cell Differentiation/genetics; RNA-Binding Proteins/metabolism; Fertility/genetics; DNA-Binding Proteins/metabolism; Cell Proliferation/genetics; Cell Cycle/genetics; Male; Gene Regulatory Networks}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{1--14}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans}},
  url          = {{http://dx.doi.org/10.1038/s41467-024-51212-0}},
  doi          = {{10.1038/s41467-024-51212-0}},
  volume       = {{15}},
  year         = {{2024}},
}