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Demonstration-scale enzymatic saccharification of sulfite-pulped spruce with addition of hydrogen peroxide for LPMO activation

Costa, Thales H.F. ; Kadic', Adnan LU ; Chylenski, Piotr ; Várnai, Anikó ; Bengtsson, Oskar ; Lidén, Gunnar LU ; Eijsink, Vincent G.H. and Horn, Svein Jarle (2020) In Biofuels, Bioproducts and Biorefining 14(4). p.734-745
Abstract

The saccharification of lignocellulosic materials like Norway spruce is challenging due to the recalcitrant nature of the biomass, and it requires optimized and efficient pretreatment and enzymatic hydrolysis processes to make it industrially feasible. In this study, we report successful enzymatic saccharification of sulfite-pulped spruce (Borregaard's BALI™ process) at demonstration scale, achieved through the controlled delivery of hydrogen peroxide (H2O2) for the activation of lytic polysaccharide monooxygenases (LPMOs) present in the cellulolytic enzyme preparation. We achieved 85% saccharification yield in 4 days using industrially relevant conditions – that is, an enzyme dose of 4% (w/w dry matter of... (More)

The saccharification of lignocellulosic materials like Norway spruce is challenging due to the recalcitrant nature of the biomass, and it requires optimized and efficient pretreatment and enzymatic hydrolysis processes to make it industrially feasible. In this study, we report successful enzymatic saccharification of sulfite-pulped spruce (Borregaard's BALI™ process) at demonstration scale, achieved through the controlled delivery of hydrogen peroxide (H2O2) for the activation of lytic polysaccharide monooxygenases (LPMOs) present in the cellulolytic enzyme preparation. We achieved 85% saccharification yield in 4 days using industrially relevant conditions – that is, an enzyme dose of 4% (w/w dry matter of substrate) of the commercial cellulase cocktail Cellic CTec3 and a substrate loading of 12% (w/w). Addition of H2O2 and the resulting controlled and high LPMO activity had a positive effect on the rate of saccharification and the final sugar titer. Clearly, the high LPMO activity was dependent on feeding the reactors with the LPMO co-substrate H2O2, as in situ generation of H2O2 from molecular oxygen was limited. These demonstration-scale experiments provide a solid basis for the use of H2O2 to improve enzymatic saccharification of lignocellulosic biomass at large industrial scale.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biorefining, cellulases, cellulose, hydrogen peroxide, LPMO, lytic polysaccharide monooxygenase, Norway spruce
in
Biofuels, Bioproducts and Biorefining
volume
14
issue
4
pages
12 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85084040502
ISSN
1932-104X
DOI
10.1002/bbb.2103
language
English
LU publication?
yes
id
d2f4130f-fdcf-4119-9e07-4b7d505ee692
date added to LUP
2020-05-28 13:22:27
date last changed
2023-12-18 21:23:52
@article{d2f4130f-fdcf-4119-9e07-4b7d505ee692,
  abstract     = {{<p>The saccharification of lignocellulosic materials like Norway spruce is challenging due to the recalcitrant nature of the biomass, and it requires optimized and efficient pretreatment and enzymatic hydrolysis processes to make it industrially feasible. In this study, we report successful enzymatic saccharification of sulfite-pulped spruce (Borregaard's BALI™ process) at demonstration scale, achieved through the controlled delivery of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for the activation of lytic polysaccharide monooxygenases (LPMOs) present in the cellulolytic enzyme preparation. We achieved 85% saccharification yield in 4 days using industrially relevant conditions – that is, an enzyme dose of 4% (w/w dry matter of substrate) of the commercial cellulase cocktail Cellic CTec3 and a substrate loading of 12% (w/w). Addition of H<sub>2</sub>O<sub>2</sub> and the resulting controlled and high LPMO activity had a positive effect on the rate of saccharification and the final sugar titer. Clearly, the high LPMO activity was dependent on feeding the reactors with the LPMO co-substrate H<sub>2</sub>O<sub>2</sub>, as in situ generation of H<sub>2</sub>O<sub>2</sub> from molecular oxygen was limited. These demonstration-scale experiments provide a solid basis for the use of H<sub>2</sub>O<sub>2</sub> to improve enzymatic saccharification of lignocellulosic biomass at large industrial scale.</p>}},
  author       = {{Costa, Thales H.F. and Kadic', Adnan and Chylenski, Piotr and Várnai, Anikó and Bengtsson, Oskar and Lidén, Gunnar and Eijsink, Vincent G.H. and Horn, Svein Jarle}},
  issn         = {{1932-104X}},
  keywords     = {{biorefining; cellulases; cellulose; hydrogen peroxide; LPMO; lytic polysaccharide monooxygenase; Norway spruce}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{734--745}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biofuels, Bioproducts and Biorefining}},
  title        = {{Demonstration-scale enzymatic saccharification of sulfite-pulped spruce with addition of hydrogen peroxide for LPMO activation}},
  url          = {{http://dx.doi.org/10.1002/bbb.2103}},
  doi          = {{10.1002/bbb.2103}},
  volume       = {{14}},
  year         = {{2020}},
}