Transplante de fração total de células mononucleares ou fração vascular estromal associada à membrana celulósica em feridas cutâneas experimentais de coelhos
(2011) In Brazilian Journal of Veterinary Research and Animal Science 48(1). p.62-72- Abstract
The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow... (More)
The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow (BM) or stromal vascular fraction (SVF) from adipose tissue (AT), associated with the use of cellulose membrane. To carry out this experiment, 20 rabbits were randomly divided in four groups with five animals each. After induction of the experimental cutaneous wound, Group A did not receive any treatment; group B received only cellulose membrane; and groups C and D, in addition to receiving the membrane, were submitted to autologous transplant of TMCF, with total cell value between 6,92 x 106 and 4,91 x 107 and a viability of 82 to 97% or SVF, with total cell value between 9,6 x 105 and 6,5 x 106 and a viability of 66 a 87%, respectively. At the end of the evaluation period, the three treated groups presented significant statistical difference of wound area in relation to the control group, and the group which received SVF from adipose tissue presented the shortest wound healing time.
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- author
- Treichel, Tiago Luis Eilers ; da Cunha, Marina Gabriela Monteiro Carvalho Mori ; da Cunha, João Paulo Monteiro Carvalho Mori LU ; Santos, Eduardo de Bastos ; Leme, Paulo de Tarso de Oliveira ; Costa, Márcio Machado ; Pippi, Ney Luis ; Lopes, Sônia Teresinha dos Anjos ; Fighera, Rafael Almeida and da Rosa, Maurício Borges
- alternative title
- Total fraction of mononuclear cell or stromal vascular fraction transplant associated with cellulosic membrane in experimental cutaneous wounds in rabbits
- publishing date
- 2011-05-31
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fraction of cells, Rabbit, Repair, Skin, Wound
- in
- Brazilian Journal of Veterinary Research and Animal Science
- volume
- 48
- issue
- 1
- pages
- 11 pages
- publisher
- Faculdade de Medicina Veterinaria e Zootecnia, USP
- external identifiers
-
- scopus:79957558033
- ISSN
- 1413-9596
- language
- Portuguese
- LU publication?
- no
- id
- d3a8d875-e159-4811-8cd1-05ba2adab082
- alternative location
- http://www.revistas.usp.br/bjvras/article/view/34377/37115
- date added to LUP
- 2019-09-18 13:50:05
- date last changed
- 2022-02-01 00:46:34
@article{d3a8d875-e159-4811-8cd1-05ba2adab082, abstract = {{<p>The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow (BM) or stromal vascular fraction (SVF) from adipose tissue (AT), associated with the use of cellulose membrane. To carry out this experiment, 20 rabbits were randomly divided in four groups with five animals each. After induction of the experimental cutaneous wound, Group A did not receive any treatment; group B received only cellulose membrane; and groups C and D, in addition to receiving the membrane, were submitted to autologous transplant of TMCF, with total cell value between 6,92 x 10<sup>6</sup> and 4,91 x 107 and a viability of 82 to 97% or SVF, with total cell value between 9,6 x 10<sup>5</sup> and 6,5 x 10<sup>6</sup> and a viability of 66 a 87%, respectively. At the end of the evaluation period, the three treated groups presented significant statistical difference of wound area in relation to the control group, and the group which received SVF from adipose tissue presented the shortest wound healing time.</p>}}, author = {{Treichel, Tiago Luis Eilers and da Cunha, Marina Gabriela Monteiro Carvalho Mori and da Cunha, João Paulo Monteiro Carvalho Mori and Santos, Eduardo de Bastos and Leme, Paulo de Tarso de Oliveira and Costa, Márcio Machado and Pippi, Ney Luis and Lopes, Sônia Teresinha dos Anjos and Fighera, Rafael Almeida and da Rosa, Maurício Borges}}, issn = {{1413-9596}}, keywords = {{Fraction of cells; Rabbit; Repair; Skin; Wound}}, language = {{por}}, month = {{05}}, number = {{1}}, pages = {{62--72}}, publisher = {{Faculdade de Medicina Veterinaria e Zootecnia, USP}}, series = {{Brazilian Journal of Veterinary Research and Animal Science}}, title = {{Transplante de fração total de células mononucleares ou fração vascular estromal associada à membrana celulósica em feridas cutâneas experimentais de coelhos}}, url = {{http://www.revistas.usp.br/bjvras/article/view/34377/37115}}, volume = {{48}}, year = {{2011}}, }