Increased gut permeability to FITC-dextran 70,000, induced by TPN, is reversed by parenteral ampicillin treatment
(1990) In Surgical Research Communications 8(1). p.7-12- Abstract
Previous experiments by our group have demonstrated that total parenteral nutrition (TPN) in rats increases the gut permeability to FITC-dextran 70,000 when compared to normally fed controls. TPN may thus, by inducing a 'leaky' gut be one co-factor behind the development of 'gut origin sepsis', a recent hypothesis for the development of multiple organ failure (MOF) in patients subjected to severe trauma or complicated surgical disease. Another co-factor behind the development of a damaged gut mucosal barrier under such conditions is a decreased 'colonization resistance' of the gut versus colonization with nosocomial microbial pathogens, a process known to be enhanced by the use of broad-spectrum antibiotics. We therefore studied the... (More)
Previous experiments by our group have demonstrated that total parenteral nutrition (TPN) in rats increases the gut permeability to FITC-dextran 70,000 when compared to normally fed controls. TPN may thus, by inducing a 'leaky' gut be one co-factor behind the development of 'gut origin sepsis', a recent hypothesis for the development of multiple organ failure (MOF) in patients subjected to severe trauma or complicated surgical disease. Another co-factor behind the development of a damaged gut mucosal barrier under such conditions is a decreased 'colonization resistance' of the gut versus colonization with nosocomial microbial pathogens, a process known to be enhanced by the use of broad-spectrum antibiotics. We therefore studied the influence of intravenously administered ampicillin on the gut permeability to FITC-dextran 70,000 after 5 days of TPN in rats and found, quite contrary to our expectations, that ampicillin normalized the TPN-induced increased permeability and that no difference could be demonstrated between normally fed rats and TPN-rats given ampicillin, whereas TPN alone significantly increased the absorption of FITC-dextran into the systemic circulation. This finding points at a role for the intestinal flora at the mucosal level in the pathogenesis of the 'leaky' of TPN and may further have an important implication on the interpretation of TPN-experiments in rats in regard to the situation in humans.
(Less)
- author
- Purandare, S. ; Offenbartl, K. ; Westrom, B. LU and Bengmark, S. LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ampicillin, FITC-dextran, gut permeability, TPN
- in
- Surgical Research Communications
- volume
- 8
- issue
- 1
- pages
- 6 pages
- publisher
- M. Gordan and Breach - Harwood Academic
- external identifiers
-
- scopus:0025328830
- ISSN
- 0882-9233
- language
- English
- LU publication?
- yes
- id
- d47d4a1f-b36e-4f79-87f7-66177718bddd
- date added to LUP
- 2024-12-05 15:30:10
- date last changed
- 2025-04-04 15:01:21
@article{d47d4a1f-b36e-4f79-87f7-66177718bddd,
abstract = {{<p>Previous experiments by our group have demonstrated that total parenteral nutrition (TPN) in rats increases the gut permeability to FITC-dextran 70,000 when compared to normally fed controls. TPN may thus, by inducing a 'leaky' gut be one co-factor behind the development of 'gut origin sepsis', a recent hypothesis for the development of multiple organ failure (MOF) in patients subjected to severe trauma or complicated surgical disease. Another co-factor behind the development of a damaged gut mucosal barrier under such conditions is a decreased 'colonization resistance' of the gut versus colonization with nosocomial microbial pathogens, a process known to be enhanced by the use of broad-spectrum antibiotics. We therefore studied the influence of intravenously administered ampicillin on the gut permeability to FITC-dextran 70,000 after 5 days of TPN in rats and found, quite contrary to our expectations, that ampicillin normalized the TPN-induced increased permeability and that no difference could be demonstrated between normally fed rats and TPN-rats given ampicillin, whereas TPN alone significantly increased the absorption of FITC-dextran into the systemic circulation. This finding points at a role for the intestinal flora at the mucosal level in the pathogenesis of the 'leaky' of TPN and may further have an important implication on the interpretation of TPN-experiments in rats in regard to the situation in humans.</p>}},
author = {{Purandare, S. and Offenbartl, K. and Westrom, B. and Bengmark, S.}},
issn = {{0882-9233}},
keywords = {{ampicillin; FITC-dextran; gut permeability; TPN}},
language = {{eng}},
number = {{1}},
pages = {{7--12}},
publisher = {{M. Gordan and Breach - Harwood Academic}},
series = {{Surgical Research Communications}},
title = {{Increased gut permeability to FITC-dextran 70,000, induced by TPN, is reversed by parenteral ampicillin treatment}},
volume = {{8}},
year = {{1990}},
}