Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury
(2018) In Blood Advances 2(13). p.1651-1663- Abstract
Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S... (More)
Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.
(Less)
- author
- organization
- publishing date
- 2018-07-10
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood Advances
- volume
- 2
- issue
- 13
- pages
- 1651 - 1663
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:29991496
- scopus:85055233556
- ISSN
- 2473-9529
- DOI
- 10.1182/bloodadvances.2018018903
- language
- English
- LU publication?
- yes
- id
- d6cb0f0d-9f0d-4abd-9b45-fc05b997f481
- date added to LUP
- 2018-08-06 16:24:36
- date last changed
- 2024-09-17 00:58:45
@article{d6cb0f0d-9f0d-4abd-9b45-fc05b997f481, abstract = {{<p>Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.</p>}}, author = {{Kapur, Rick and Kim, Michael and Rebetz, Johan and Hallström, Björn and Björkman, Jonas T and Takabe-French, Alisa and Kim, Noel and Liu, Jonathan and Shanmugabhavananthan, Shanjeevan and Milosevic, Stefan and McVey, Mark J and Speck, Edwin R and Semple, John W}}, issn = {{2473-9529}}, language = {{eng}}, month = {{07}}, number = {{13}}, pages = {{1651--1663}}, publisher = {{American Society of Hematology}}, series = {{Blood Advances}}, title = {{Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury}}, url = {{http://dx.doi.org/10.1182/bloodadvances.2018018903}}, doi = {{10.1182/bloodadvances.2018018903}}, volume = {{2}}, year = {{2018}}, }