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Acquisition of neural fate by combination of BMP blockade and chromatin modification

Ong, Agnes Lee Chen LU orcid ; Kokaji, Toshiya ; Kishi, Arisa ; Takihara, Yoshihiro ; Shinozuka, Takuma ; Shimamoto, Ren ; Isotani, Ayako ; Shirai, Manabu and Sasai, Noriaki (2023) In iScience 26(10). p.1-17
Abstract

Neural induction is a process where naive cells are converted into committed cells with neural characteristics, and it occurs at the earliest step during embryogenesis. Although the signaling molecules and chromatin remodeling for neural induction have been identified, the mutual relationships between these molecules are yet to be fully understood. By taking advantage of the neural differentiation system of mouse embryonic stem (ES) cells, we discovered that the BMP signal regulates the expression of several polycomb repressor complex (PRC) component genes. We particularly focused on Polyhomeotic Homolog 1 (Phc1) and established Phc1-knockout (Phc1-KO) ES cells. We found that Phc1-KO failed to acquire the neural fate, and the cells... (More)

Neural induction is a process where naive cells are converted into committed cells with neural characteristics, and it occurs at the earliest step during embryogenesis. Although the signaling molecules and chromatin remodeling for neural induction have been identified, the mutual relationships between these molecules are yet to be fully understood. By taking advantage of the neural differentiation system of mouse embryonic stem (ES) cells, we discovered that the BMP signal regulates the expression of several polycomb repressor complex (PRC) component genes. We particularly focused on Polyhomeotic Homolog 1 (Phc1) and established Phc1-knockout (Phc1-KO) ES cells. We found that Phc1-KO failed to acquire the neural fate, and the cells remained in pluripotent or primitive non-neural states. Chromatin accessibility analysis suggests that Phc1 is essential for chromatin packing. Aberrant upregulation of the BMP signal was confirmed in the Phc1 homozygotic mutant embryos. Taken together, Phc1 is required for neural differentiation through epigenetic modification.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Epigenetics, Molecular biology, Neuroscience, Stem cells research, Transcriptomics
in
iScience
volume
26
issue
10
article number
107887
pages
1 - 17
publisher
Elsevier
external identifiers
  • scopus:85172329872
ISSN
2589-0042
DOI
10.1016/j.isci.2023.107887
language
English
LU publication?
no
additional info
Publisher Copyright: © 2023 The Authors
id
d8a5d221-967e-4d15-a031-565b4c009bb3
date added to LUP
2026-01-23 23:17:37
date last changed
2026-01-26 07:14:48
@article{d8a5d221-967e-4d15-a031-565b4c009bb3,
  abstract     = {{<p>Neural induction is a process where naive cells are converted into committed cells with neural characteristics, and it occurs at the earliest step during embryogenesis. Although the signaling molecules and chromatin remodeling for neural induction have been identified, the mutual relationships between these molecules are yet to be fully understood. By taking advantage of the neural differentiation system of mouse embryonic stem (ES) cells, we discovered that the BMP signal regulates the expression of several polycomb repressor complex (PRC) component genes. We particularly focused on Polyhomeotic Homolog 1 (Phc1) and established Phc1-knockout (Phc1-KO) ES cells. We found that Phc1-KO failed to acquire the neural fate, and the cells remained in pluripotent or primitive non-neural states. Chromatin accessibility analysis suggests that Phc1 is essential for chromatin packing. Aberrant upregulation of the BMP signal was confirmed in the Phc1 homozygotic mutant embryos. Taken together, Phc1 is required for neural differentiation through epigenetic modification.</p>}},
  author       = {{Ong, Agnes Lee Chen and Kokaji, Toshiya and Kishi, Arisa and Takihara, Yoshihiro and Shinozuka, Takuma and Shimamoto, Ren and Isotani, Ayako and Shirai, Manabu and Sasai, Noriaki}},
  issn         = {{2589-0042}},
  keywords     = {{Epigenetics; Molecular biology; Neuroscience; Stem cells research; Transcriptomics}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{10}},
  pages        = {{1--17}},
  publisher    = {{Elsevier}},
  series       = {{iScience}},
  title        = {{Acquisition of neural fate by combination of BMP blockade and chromatin modification}},
  url          = {{http://dx.doi.org/10.1016/j.isci.2023.107887}},
  doi          = {{10.1016/j.isci.2023.107887}},
  volume       = {{26}},
  year         = {{2023}},
}