Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry

Ertürk, Gizem LU ; Akhoundian, Maedeh ; Lueg-Althoff, Kyra ; Shinde, Sudhirkumar ; Yeung, Sing Yee ; Hedström, Martin LU ; Schrader, Thomas ; Mattiasson, Bo LU and Sellergren, Börje (2018) In New Journal of Chemistry 43(2). p.847-852
Abstract

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We... (More)

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Journal of Chemistry
volume
43
issue
2
pages
6 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85059570875
ISSN
1144-0546
DOI
10.1039/c8nj05238g
language
English
LU publication?
yes
id
d92e068e-f783-4d9c-8656-768604783cb9
date added to LUP
2019-01-18 14:14:05
date last changed
2022-04-18 01:46:07
@article{d92e068e-f783-4d9c-8656-768604783cb9,
  abstract     = {{<p>Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.</p>}},
  author       = {{Ertürk, Gizem and Akhoundian, Maedeh and Lueg-Althoff, Kyra and Shinde, Sudhirkumar and Yeung, Sing Yee and Hedström, Martin and Schrader, Thomas and Mattiasson, Bo and Sellergren, Börje}},
  issn         = {{1144-0546}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{2}},
  pages        = {{847--852}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{New Journal of Chemistry}},
  title        = {{Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry}},
  url          = {{http://dx.doi.org/10.1039/c8nj05238g}},
  doi          = {{10.1039/c8nj05238g}},
  volume       = {{43}},
  year         = {{2018}},
}