Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry
(2018) In New Journal of Chemistry 43(2). p.847-852- Abstract
Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We... (More)
Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.
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- author
- Ertürk, Gizem LU ; Akhoundian, Maedeh ; Lueg-Althoff, Kyra ; Shinde, Sudhirkumar ; Yeung, Sing Yee ; Hedström, Martin LU ; Schrader, Thomas ; Mattiasson, Bo LU and Sellergren, Börje
- organization
- publishing date
- 2018-12-04
- type
- Contribution to journal
- publication status
- published
- subject
- in
- New Journal of Chemistry
- volume
- 43
- issue
- 2
- pages
- 6 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85059570875
- ISSN
- 1144-0546
- DOI
- 10.1039/c8nj05238g
- language
- English
- LU publication?
- yes
- id
- d92e068e-f783-4d9c-8656-768604783cb9
- date added to LUP
- 2019-01-18 14:14:05
- date last changed
- 2022-04-18 01:46:07
@article{d92e068e-f783-4d9c-8656-768604783cb9, abstract = {{<p>Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the specific interaction between bisphosphonate binding monomers and lysine/arginine residues on the surface of trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.</p>}}, author = {{Ertürk, Gizem and Akhoundian, Maedeh and Lueg-Althoff, Kyra and Shinde, Sudhirkumar and Yeung, Sing Yee and Hedström, Martin and Schrader, Thomas and Mattiasson, Bo and Sellergren, Börje}}, issn = {{1144-0546}}, language = {{eng}}, month = {{12}}, number = {{2}}, pages = {{847--852}}, publisher = {{Royal Society of Chemistry}}, series = {{New Journal of Chemistry}}, title = {{Bisphosphonate ligand mediated ultrasensitive capacitive protein sensor : complementary match of supramolecular and dynamic chemistry}}, url = {{http://dx.doi.org/10.1039/c8nj05238g}}, doi = {{10.1039/c8nj05238g}}, volume = {{43}}, year = {{2018}}, }