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Synthesis of 2-acetamido-2-deoxy-3-O-β-d-galactopyrano-syl-d-galactose by the sequential use of β-d-galactosidases from bovine testes and escherichia coli

Hedbys, Lars ; Johansson, Elisabet ; Mosbach, Klaus LU ; Larsson, Per-Olof LU ; Gunnarsson, Alf and Svensson, Sigfrid (1989) In Carbohydrate Research 186(2). p.217-223
Abstract
β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the GalNAc added.
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
β-D-Galp-(1→3)-D-GalNAc, Transgalactosylation, β-galactosidase, E. coli, Bovine testes
in
Carbohydrate Research
volume
186
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0024969887
ISSN
0008-6215
DOI
10.1016/0008-6215(89)84036-4
language
English
LU publication?
yes
id
e08417f1-7c0a-492e-86b0-c0a778fe4d32
date added to LUP
2024-06-17 14:53:16
date last changed
2024-09-02 15:16:23
@article{e08417f1-7c0a-492e-86b0-c0a778fe4d32,
  abstract     = {{β-d-Galp-(1→3)-d-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using β-d-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with β-d-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the GalNAc added.}},
  author       = {{Hedbys, Lars and Johansson, Elisabet and Mosbach, Klaus and Larsson, Per-Olof and Gunnarsson, Alf and Svensson, Sigfrid}},
  issn         = {{0008-6215}},
  keywords     = {{β-D-Galp-(1→3)-D-GalNAc; Transgalactosylation; β-galactosidase; E. coli; Bovine testes}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{217--223}},
  publisher    = {{Elsevier}},
  series       = {{Carbohydrate Research}},
  title        = {{Synthesis of 2-acetamido-2-deoxy-3-O-β-d-galactopyrano-syl-d-galactose by the sequential use of β-d-galactosidases from bovine testes and <i>escherichia coli</i>}},
  url          = {{http://dx.doi.org/10.1016/0008-6215(89)84036-4}},
  doi          = {{10.1016/0008-6215(89)84036-4}},
  volume       = {{186}},
  year         = {{1989}},
}