Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Control of an affinity purification procedure using a thermal biosensor

Flygare, Lilian ; Larsson, Per-Olof LU and Danielsson, Bengt LU (1990) In Biotechnology and Bioengineering 36(7). p.723-726
Abstract
Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6-(6-aminohexyl)-AMP-Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP-Sepharose gel to the LDH solution. In another type of experiment, a desktop computer was utilized to control the addition of the adsorbent. Both systems worked satisfactorily, and enabled a rapid and accurate assessment of correct addition of adsorbent.
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme thermistor, Affinity purification
in
Biotechnology and Bioengineering
volume
36
issue
7
pages
4 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0025701091
ISSN
1097-0290
DOI
10.1002/bit.260360710
language
English
LU publication?
yes
id
e1bd07e7-a19f-4c7d-8aee-e47ff330f7ca
date added to LUP
2024-06-17 13:47:11
date last changed
2025-10-14 10:49:31
@article{e1bd07e7-a19f-4c7d-8aee-e47ff330f7ca,
  abstract     = {{Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6-(6-aminohexyl)-AMP-Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP-Sepharose gel to the LDH solution. In another type of experiment, a desktop computer was utilized to control the addition of the adsorbent. Both systems worked satisfactorily, and enabled a rapid and accurate assessment of correct addition of adsorbent.}},
  author       = {{Flygare, Lilian and Larsson, Per-Olof and Danielsson, Bengt}},
  issn         = {{1097-0290}},
  keywords     = {{Enzyme thermistor; Affinity purification}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{723--726}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Control of an affinity purification procedure using a thermal biosensor}},
  url          = {{http://dx.doi.org/10.1002/bit.260360710}},
  doi          = {{10.1002/bit.260360710}},
  volume       = {{36}},
  year         = {{1990}},
}