Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

γ-Aminobutyric acid activation of 36Cl-flux in rat hippocampal slices and its potentiation by barbiturates

Wong, Erik H.F. ; Leeb-Lundberg, L. M.Fredrik LU ; Teichberg, Vivian I. and Olsen, Richard W. (1984) In Brain Research 303(2). p.267-275
Abstract

γ-Aminobutyric acid (GABA) increases the rate of 36Cl- efflux from preloaded rat hippocampal slices in a dose-dependent manner (EC50: 400 μM). This action has the pharmacological specificity expected of activation of GABA receptor in that it is mimicked by the agonists muscimol and 3-aminopropanesulfonic acid, and blocked by the antagonists bicuculline and picrotoxinin. GABA uptake inhibitors, nipecotic acid and 2,4-diaminobutyric acid, fail to increase 36Cl- flux. Pentobarbital produces a dose-dependent activation (EC50 = 1.5 mM) of 36Cl- efflux with maximal response greater than that of GABA. The effect of pentobarbital can be mimicked by 1,3-dimethylbutylbarbiturate,... (More)

γ-Aminobutyric acid (GABA) increases the rate of 36Cl- efflux from preloaded rat hippocampal slices in a dose-dependent manner (EC50: 400 μM). This action has the pharmacological specificity expected of activation of GABA receptor in that it is mimicked by the agonists muscimol and 3-aminopropanesulfonic acid, and blocked by the antagonists bicuculline and picrotoxinin. GABA uptake inhibitors, nipecotic acid and 2,4-diaminobutyric acid, fail to increase 36Cl- flux. Pentobarbital produces a dose-dependent activation (EC50 = 1.5 mM) of 36Cl- efflux with maximal response greater than that of GABA. The effect of pentobarbital can be mimicked by 1,3-dimethylbutylbarbiturate, secobarbital, (+)hexobarbital but not (-)hexobarbital, and is blocked by bicuculline and picrotoxinin. Pentobarbital and the other active barbiturates also potentiate the action of GABA. Phenobarbital does not have any effect independently or in combination with GABA. It is suggested that GABA increases 36Cl- permeability by activation of a postsynaptic receptor which is in turn functionally copupled to a barbiturate receptor.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
barbiturates, chloride channels, hippocampal slices, inhibitory synapse, postsynaptic receptors, γ-aminobutyric acid
in
Brain Research
volume
303
issue
2
pages
267 - 275
publisher
Elsevier
external identifiers
  • scopus:0021215487
  • pmid:6331574
ISSN
0006-8993
DOI
10.1016/0006-8993(84)91213-7
language
English
LU publication?
no
id
e2f36013-0496-41e2-a23c-201d40e7ebd4
date added to LUP
2019-06-12 11:51:07
date last changed
2025-04-04 15:12:49
@article{e2f36013-0496-41e2-a23c-201d40e7ebd4,
  abstract     = {{<p>γ-Aminobutyric acid (GABA) increases the rate of <sup>36</sup>Cl- efflux from preloaded rat hippocampal slices in a dose-dependent manner (EC<sub>50</sub>: 400 μM). This action has the pharmacological specificity expected of activation of GABA receptor in that it is mimicked by the agonists muscimol and 3-aminopropanesulfonic acid, and blocked by the antagonists bicuculline and picrotoxinin. GABA uptake inhibitors, nipecotic acid and 2,4-diaminobutyric acid, fail to increase <sup>36</sup>Cl- flux. Pentobarbital produces a dose-dependent activation (EC<sub>50</sub> = 1.5 mM) of <sup>36</sup>Cl- efflux with maximal response greater than that of GABA. The effect of pentobarbital can be mimicked by 1,3-dimethylbutylbarbiturate, secobarbital, (+)hexobarbital but not (-)hexobarbital, and is blocked by bicuculline and picrotoxinin. Pentobarbital and the other active barbiturates also potentiate the action of GABA. Phenobarbital does not have any effect independently or in combination with GABA. It is suggested that GABA increases <sup>36</sup>Cl- permeability by activation of a postsynaptic receptor which is in turn functionally copupled to a barbiturate receptor.</p>}},
  author       = {{Wong, Erik H.F. and Leeb-Lundberg, L. M.Fredrik and Teichberg, Vivian I. and Olsen, Richard W.}},
  issn         = {{0006-8993}},
  keywords     = {{barbiturates; chloride channels; hippocampal slices; inhibitory synapse; postsynaptic receptors; γ-aminobutyric acid}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{2}},
  pages        = {{267--275}},
  publisher    = {{Elsevier}},
  series       = {{Brain Research}},
  title        = {{γ-Aminobutyric acid activation of <sup>36</sup>Cl-flux in rat hippocampal slices and its potentiation by barbiturates}},
  url          = {{http://dx.doi.org/10.1016/0006-8993(84)91213-7}},
  doi          = {{10.1016/0006-8993(84)91213-7}},
  volume       = {{303}},
  year         = {{1984}},
}