Protease activity of Blastocystis hominis
(2006) In Parasitology Research 99(2). p.30-126- Abstract
Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.
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https://lup.lub.lu.se/record/e33325a2-3fe7-4858-80a6-9c0c4b270dc5
- author
- Sio, Selena W S ; Puthia, Manoj K LU ; Lee, Alex S Y ; Lu, Jia and Tan, Kevin S W
- publishing date
- 2006-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Blastocystis hominis/drug effects, Cysteine Endopeptidases/metabolism, Electrophoresis, Polyacrylamide Gel, Humans, Peptide Hydrolases/drug effects, Protease Inhibitors/pharmacology
- in
- Parasitology Research
- volume
- 99
- issue
- 2
- pages
- 30 - 126
- publisher
- Springer
- external identifiers
-
- pmid:16518611
- scopus:33745044249
- ISSN
- 0932-0113
- DOI
- 10.1007/s00436-006-0131-1
- language
- English
- LU publication?
- no
- id
- e33325a2-3fe7-4858-80a6-9c0c4b270dc5
- date added to LUP
- 2018-08-27 13:44:20
- date last changed
- 2024-08-05 21:19:37
@article{e33325a2-3fe7-4858-80a6-9c0c4b270dc5, abstract = {{<p>Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.</p>}}, author = {{Sio, Selena W S and Puthia, Manoj K and Lee, Alex S Y and Lu, Jia and Tan, Kevin S W}}, issn = {{0932-0113}}, keywords = {{Animals; Blastocystis hominis/drug effects; Cysteine Endopeptidases/metabolism; Electrophoresis, Polyacrylamide Gel; Humans; Peptide Hydrolases/drug effects; Protease Inhibitors/pharmacology}}, language = {{eng}}, number = {{2}}, pages = {{30--126}}, publisher = {{Springer}}, series = {{Parasitology Research}}, title = {{Protease activity of Blastocystis hominis}}, url = {{http://dx.doi.org/10.1007/s00436-006-0131-1}}, doi = {{10.1007/s00436-006-0131-1}}, volume = {{99}}, year = {{2006}}, }