Development of a novel ex vivo model for chemical ocular toxicity assessment and its applicability for hair straightening products
(2022) In Food and Chemical Toxicology 170.- Abstract
This study developed an air-liquid interface (ALI) corneal model using explants bovine eyes for ocular toxicity assessment of ten chemicals and seven hair straightening mixtures. It was successfully maintained physiologically viable and normal for six days. Both eye damage (GHS cat. 1) and irritating (GHS cat. 2) chemicals induced corneal injury in our model. However, cat. 2 irritants triggered moderate damage when compared to cat. 1 agents, which induced a marked cytotoxicity profile. The mixtures were also able to trigger viability reduction associated with histopathological changes in the corneal tissues, especially when the exposure was via aerosol particles. Thus, the chemical exposure microenvironment simulation seemed to provide... (More)
This study developed an air-liquid interface (ALI) corneal model using explants bovine eyes for ocular toxicity assessment of ten chemicals and seven hair straightening mixtures. It was successfully maintained physiologically viable and normal for six days. Both eye damage (GHS cat. 1) and irritating (GHS cat. 2) chemicals induced corneal injury in our model. However, cat. 2 irritants triggered moderate damage when compared to cat. 1 agents, which induced a marked cytotoxicity profile. The mixtures were also able to trigger viability reduction associated with histopathological changes in the corneal tissues, especially when the exposure was via aerosol particles. Thus, the chemical exposure microenvironment simulation seemed to provide more reliable toxicological data. Moreover, mixture-induced corneal damage correlated with increased ROS levels, suggesting a close correlation between tissue death and oxidative stress. Besides mixtures showing the potential to induce moderate/mild ocular toxicity, we could verify that the corneal tissue damage showed reversibility due to the recovery from the injury after exposure to some of the mixtures. Hence, our ex vivo corneal model seems to be a simple and cost-effective approach for future studies related to further investigating the reversibility of damage in the cornea triggered by chemicals and their mixtures.
(Less)
- author
- Rodrigues da Penha, Jaqueline ; Garcia da Silva, Artur Christian ; de Ávila, Renato Ivan LU and Valadares, Marize Campos
- organization
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Air-liquid interface, Cosmetics, Eye damage, Mixtures, Nebulization exposure, New approach methods, Ocular irritation
- in
- Food and Chemical Toxicology
- volume
- 170
- article number
- 113457
- publisher
- Elsevier
- external identifiers
-
- scopus:85140742209
- pmid:36244458
- ISSN
- 0278-6915
- DOI
- 10.1016/j.fct.2022.113457
- language
- English
- LU publication?
- yes
- id
- e4e375e6-673d-45d2-b6a7-cd894d0f557e
- date added to LUP
- 2022-12-06 12:31:28
- date last changed
- 2024-07-12 01:29:10
@article{e4e375e6-673d-45d2-b6a7-cd894d0f557e,
abstract = {{<p>This study developed an air-liquid interface (ALI) corneal model using explants bovine eyes for ocular toxicity assessment of ten chemicals and seven hair straightening mixtures. It was successfully maintained physiologically viable and normal for six days. Both eye damage (GHS cat. 1) and irritating (GHS cat. 2) chemicals induced corneal injury in our model. However, cat. 2 irritants triggered moderate damage when compared to cat. 1 agents, which induced a marked cytotoxicity profile. The mixtures were also able to trigger viability reduction associated with histopathological changes in the corneal tissues, especially when the exposure was via aerosol particles. Thus, the chemical exposure microenvironment simulation seemed to provide more reliable toxicological data. Moreover, mixture-induced corneal damage correlated with increased ROS levels, suggesting a close correlation between tissue death and oxidative stress. Besides mixtures showing the potential to induce moderate/mild ocular toxicity, we could verify that the corneal tissue damage showed reversibility due to the recovery from the injury after exposure to some of the mixtures. Hence, our ex vivo corneal model seems to be a simple and cost-effective approach for future studies related to further investigating the reversibility of damage in the cornea triggered by chemicals and their mixtures.</p>}},
author = {{Rodrigues da Penha, Jaqueline and Garcia da Silva, Artur Christian and de Ávila, Renato Ivan and Valadares, Marize Campos}},
issn = {{0278-6915}},
keywords = {{Air-liquid interface; Cosmetics; Eye damage; Mixtures; Nebulization exposure; New approach methods; Ocular irritation}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Food and Chemical Toxicology}},
title = {{Development of a novel ex vivo model for chemical ocular toxicity assessment and its applicability for hair straightening products}},
url = {{http://dx.doi.org/10.1016/j.fct.2022.113457}},
doi = {{10.1016/j.fct.2022.113457}},
volume = {{170}},
year = {{2022}},
}