Convergence of chromogranin and amyloid metabolism in the brain.
(2010) Alzheimer's Association International Conference on Alzheimer's Disease 2010 In Alzheimer's & Dementia 6(4). p.511-511- Abstract
- Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII... (More)
- Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons. (Less)
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- author
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Alzheimer's & Dementia
- volume
- 6
- issue
- 4
- pages
- 511 - 511
- publisher
- Wiley
- conference name
- Alzheimer's Association International Conference on Alzheimer's Disease 2010
- conference location
- Honolulu, United States
- conference dates
- 2010-07-10 - 2010-07-15
- ISSN
- 1552-5279
- DOI
- 10.1016/j.jalz.2010.05.1705
- project
- Endocrine and diagnostic aspects of cognitive impairment
- language
- English
- LU publication?
- yes
- id
- e78cf298-f013-48de-88f5-d6679d7cb49f
- date added to LUP
- 2016-12-02 20:50:20
- date last changed
- 2023-04-18 17:53:03
@misc{e78cf298-f013-48de-88f5-d6679d7cb49f, abstract = {{Background: Much is unknown regarding the regulation of amyloid precursor protein (APP) processing in the human central nervous system. It has been hypothesized that amyloidogenic APP-processing preferentially occurs in the regulated secretory pathway of neurons. To test this hypothesis we looked for correlations of APP-derived molecules in CSF with chromogranin (Cg) derived peptides, representing the regulated secretion. Methods: Patients with Alzheimer's disease (AD, N = 32), multiple sclerosis (MS, N = 50) and healthy controls (N = 70) were enrolled. CSF was analyzed for the amyloid peptides Aβ1-42, Aβx-42, Aβx-40, Aβx-38, α-cleaved soluble APP (α-sAPP), β-cleaved soluble APP (β-sAPP), and peptides derived from CgB and SgII (Secretogranin-II, CgC). We investigated CSF levels of the protease BACE1, which processes APP into Aβ, in relation to Cg-levels. Finally, we measured Cg levels in cell media from untreated and BACE1-inhibited SH-SY5Y human neuroblastoma cells. Results: CSF Cg levels correlated to sAPP and Aβ peptides in AD, MS and controls, and to CSF BACE1. Cell medium from BACE1-inhibited cells had decreased CgB levels. Conclusions: These results suggest that a large part of APP in the human central nervous system is processed in the regulated secretory pathway of neurons.}}, author = {{Johansson, Per and Mattsson, Niklas and Hansson, Oskar and Wallin, A. and Johansson, J.-O. and Andreasson, U. and Andersen, O and Haghighi, S. and Olsson, M. and Stridsberg, M and Svensson, J. and Blennow, K. and Zetterberg, H.}}, issn = {{1552-5279}}, language = {{eng}}, note = {{Conference Abstract}}, number = {{4}}, pages = {{511--511}}, publisher = {{Wiley}}, series = {{Alzheimer's & Dementia}}, title = {{Convergence of chromogranin and amyloid metabolism in the brain.}}, url = {{http://dx.doi.org/10.1016/j.jalz.2010.05.1705}}, doi = {{10.1016/j.jalz.2010.05.1705}}, volume = {{6}}, year = {{2010}}, }