Steroid transformation by activated living immobilized Arthrobacter simplex cells
(1978) In Biotechnology and Bioengineering 20(8). p.1267-1284- Abstract
- A preparation of living Arthrobacter simplex cells immobilized in polyacrylamide gel, which showed steroid-Δ1-dehydrogenase activity, was studied. The entrapped microorganisms catalyzed the transformation of cortisol to prednisolone and this reaction was followed spectrophotometrically or with the aid of thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). About 40% of the original activity found with free bacteria was retained after immobilization. The steroid dehydrogenase activity of polyacrylamide-entrapped A. simplex could be raised to a minor extent in alcoholic solvents or by addition of a cofactor such as menadione. On incubation in various nutrient media, on the other hand, the... (More)
- A preparation of living Arthrobacter simplex cells immobilized in polyacrylamide gel, which showed steroid-Δ1-dehydrogenase activity, was studied. The entrapped microorganisms catalyzed the transformation of cortisol to prednisolone and this reaction was followed spectrophotometrically or with the aid of thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). About 40% of the original activity found with free bacteria was retained after immobilization. The steroid dehydrogenase activity of polyacrylamide-entrapped A. simplex could be raised to a minor extent in alcoholic solvents or by addition of a cofactor such as menadione. On incubation in various nutrient media, on the other hand, the activity could be increased considerablyl, usually 7–10 times. Possible causes for the observed increase in activity have been investigated, and microbial growth of the original entrapped microorganisms appears to be the major reason. Frozen activated preparations of immobilized A. simplex showed only a small loss of activity on storage for at least four months. A semicontinuous batch wise operation with immobilized A. simplex in different nutrient media was carried out. At the end of the experiment the steroid transformation capacity was 0.5 g steroid per day per g gel (wet weight). (Less)
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https://lup.lub.lu.se/record/f1322287-bf4b-4ea2-a523-bf943ade9c28
- author
- Ohlson, Sten ; Larsson, Per-Olof LU and Mosbach, Klaus LU
- organization
- publishing date
- 1978
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 20
- issue
- 8
- pages
- 18 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0017822709
- ISSN
- 0006-3592
- DOI
- 10.1002/bit.260200811
- language
- English
- LU publication?
- yes
- id
- f1322287-bf4b-4ea2-a523-bf943ade9c28
- date added to LUP
- 2024-06-19 11:33:32
- date last changed
- 2024-09-13 11:00:21
@article{f1322287-bf4b-4ea2-a523-bf943ade9c28,
abstract = {{A preparation of living <i>Arthrobacter simplex</i> cells immobilized in polyacrylamide gel, which showed steroid-Δ<sup>1</sup>-dehydrogenase activity, was studied. The entrapped microorganisms catalyzed the transformation of cortisol to prednisolone and this reaction was followed spectrophotometrically or with the aid of thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). About 40% of the original activity found with free bacteria was retained after immobilization. The steroid dehydrogenase activity of polyacrylamide-entrapped <i>A. simplex</i> could be raised to a minor extent in alcoholic solvents or by addition of a cofactor such as menadione. On incubation in various nutrient media, on the other hand, the activity could be increased considerablyl, usually 7–10 times. Possible causes for the observed increase in activity have been investigated, and microbial growth of the original entrapped microorganisms appears to be the major reason. Frozen activated preparations of immobilized <i>A. simplex</i> showed only a small loss of activity on storage for at least four months. A semicontinuous batch wise operation with immobilized <i>A. simplex</i> in different nutrient media was carried out. At the end of the experiment the steroid transformation capacity was 0.5 g steroid per day per g gel (wet weight).}},
author = {{Ohlson, Sten and Larsson, Per-Olof and Mosbach, Klaus}},
issn = {{0006-3592}},
language = {{eng}},
number = {{8}},
pages = {{1267--1284}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Steroid transformation by activated living immobilized <i>Arthrobacter simplex</i> cells}},
url = {{http://dx.doi.org/10.1002/bit.260200811}},
doi = {{10.1002/bit.260200811}},
volume = {{20}},
year = {{1978}},
}