Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection
(2019) In Fertility and Sterility 112(1). p.2-53- Abstract
Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks).... (More)
Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks). For all treatments, the odds ratio (OR)and 95% confidence interval (CI)for early miscarriage was 1.41 (1.07–1.85)if HDS >15% compared with HDS ≤15%. When comparing the two HDS categories, for ICSI, the OR was 1.44 (1.01–2.04)whereas for IVF the results were not statistically significant. Conclusion(s): There is a small but increased risk of early miscarriage if HDS >15% compared with HDS ≤15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments.
(Less)
- author
- Jerre, Elsa ; Bungum, Mona LU ; Evenson, Donald and Giwercman, Aleksander LU
- organization
- publishing date
- 2019-04-28
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DNA fragmentation, early miscarriage rate, high DNA stainability, in vitro fertilization, sperm DNA integrity
- in
- Fertility and Sterility
- volume
- 112
- issue
- 1
- pages
- 2 - 53
- publisher
- Elsevier
- external identifiers
-
- scopus:85064767329
- pmid:31043234
- ISSN
- 0015-0282
- DOI
- 10.1016/j.fertnstert.2019.03.013
- language
- English
- LU publication?
- yes
- id
- fc5f77c5-a62e-45dc-8382-bd344794b05c
- date added to LUP
- 2019-05-06 13:40:20
- date last changed
- 2024-09-17 18:48:22
@article{fc5f77c5-a62e-45dc-8382-bd344794b05c, abstract = {{<p>Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks). For all treatments, the odds ratio (OR)and 95% confidence interval (CI)for early miscarriage was 1.41 (1.07–1.85)if HDS >15% compared with HDS ≤15%. When comparing the two HDS categories, for ICSI, the OR was 1.44 (1.01–2.04)whereas for IVF the results were not statistically significant. Conclusion(s): There is a small but increased risk of early miscarriage if HDS >15% compared with HDS ≤15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments.</p>}}, author = {{Jerre, Elsa and Bungum, Mona and Evenson, Donald and Giwercman, Aleksander}}, issn = {{0015-0282}}, keywords = {{DNA fragmentation; early miscarriage rate; high DNA stainability; in vitro fertilization; sperm DNA integrity}}, language = {{eng}}, month = {{04}}, number = {{1}}, pages = {{2--53}}, publisher = {{Elsevier}}, series = {{Fertility and Sterility}}, title = {{Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection}}, url = {{http://dx.doi.org/10.1016/j.fertnstert.2019.03.013}}, doi = {{10.1016/j.fertnstert.2019.03.013}}, volume = {{112}}, year = {{2019}}, }