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Steroid transformation by living cells immobilized in calcium alginate

Ohlson, Sten ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1979) In European Journal of Applied Microbiology and Biotechnology 7. p.103-110
Abstract
Whole cells of Arthrobacter simplex were immobilized in a living state in calcium alginate gel. The bacteria showed steroid-Δ1-dehydrogenase activity and the production of prednisolone from cortisol was investigated. The Δ1-dehydrogenase activity of the immobilized cells could be increased about ten-fold by incubation in nutrient media (e.g., containing 0.5% peptone abd 0.2% glucose). The reason for this activation was examined and it was found that the immobilized cells were capable of multiplying when supplied with nutrients. Furthermore, provided that an inducer, cortisol, was present, the steroid-Δ1-dehydrogenase activity increased in proportion to the increase in the number of cells and it was... (More)
Whole cells of Arthrobacter simplex were immobilized in a living state in calcium alginate gel. The bacteria showed steroid-Δ1-dehydrogenase activity and the production of prednisolone from cortisol was investigated. The Δ1-dehydrogenase activity of the immobilized cells could be increased about ten-fold by incubation in nutrient media (e.g., containing 0.5% peptone abd 0.2% glucose). The reason for this activation was examined and it was found that the immobilized cells were capable of multiplying when supplied with nutrients. Furthermore, provided that an inducer, cortisol, was present, the steroid-Δ1-dehydrogenase activity increased in proportion to the increase in the number of cells and it was thus concluded that microbial growth was the cause of activation.

Experiments on repeated, batch-wise pseudocrystallofermentation with immobilized A. simplex cells also showed that immobilized cells could be advantageously used for pseudocrystallofermentation of steroids. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Steroid transformation, Prednisolon, Calcium alginate
in
European Journal of Applied Microbiology and Biotechnology
volume
7
pages
8 pages
publisher
Springer
external identifiers
  • scopus:0018731336
ISSN
0171-1741
DOI
10.1007/BF00505015
language
English
LU publication?
yes
id
fcded1f3-c353-466b-a0ef-b08c7b042a8b
date added to LUP
2024-06-19 10:27:05
date last changed
2025-04-04 13:51:01
@article{fcded1f3-c353-466b-a0ef-b08c7b042a8b,
  abstract     = {{Whole cells of <i>Arthrobacter simplex</i> were immobilized in a living state in calcium alginate gel. The bacteria showed steroid-Δ<sup>1</sup>-dehydrogenase activity and the production of prednisolone from cortisol was investigated. The Δ<sup>1</sup>-dehydrogenase activity of the immobilized cells could be increased about ten-fold by incubation in nutrient media (e.g., containing 0.5% peptone abd 0.2% glucose). The reason for this activation was examined and it was found that the immobilized cells were capable of multiplying when supplied with nutrients. Furthermore, provided that an inducer, cortisol, was present, the steroid-Δ<sup>1</sup>-dehydrogenase activity increased in proportion to the increase in the number of cells and it was thus concluded that microbial growth was the cause of activation.<br/><br/>Experiments on repeated, batch-wise pseudocrystallofermentation with immobilized A. simplex cells also showed that immobilized cells could be advantageously used for pseudocrystallofermentation of steroids.}},
  author       = {{Ohlson, Sten and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0171-1741}},
  keywords     = {{Steroid transformation; Prednisolon; Calcium alginate}},
  language     = {{eng}},
  pages        = {{103--110}},
  publisher    = {{Springer}},
  series       = {{European Journal of Applied Microbiology and Biotechnology}},
  title        = {{Steroid transformation by living cells immobilized in calcium alginate}},
  url          = {{http://dx.doi.org/10.1007/BF00505015}},
  doi          = {{10.1007/BF00505015}},
  volume       = {{7}},
  year         = {{1979}},
}