Spatiotemporal distribution of neural crest cells in the common wall lizard Podarcis muralis
Pranter, Robin LU
and Feiner, Nathalie
LU
(2024)
In Developmental Dynamics
- Abstract
Background: Neural crest cells (NCCs) are migratory embryonic stem cells that give rise to a diverse set of cell types. Here we describe the dynamic distribution of NCCs in developing embryos of the common wall lizard Podarcis muralis inferred from 10 markers. Our aim is to provide insights into the NCC development of lacertid lizards and to infer evolutionary modifications by comparisons to other tetrapods. Results: NCC migration is ongoing at oviposition, following three streams in the head and multiple in the trunk. From 21ss, we observe expression patterns indicating the beginning of differentiation toward mesenchymal and neuronal fates. By 35ss, migration is restricted to caudal levels, and fully differentiated chromaffin cells are... (More)
Background: Neural crest cells (NCCs) are migratory embryonic stem cells that give rise to a diverse set of cell types. Here we describe the dynamic distribution of NCCs in developing embryos of the common wall lizard Podarcis muralis inferred from 10 markers. Our aim is to provide insights into the NCC development of lacertid lizards and to infer evolutionary modifications by comparisons to other tetrapods. Results: NCC migration is ongoing at oviposition, following three streams in the head and multiple in the trunk. From 21ss, we observe expression patterns indicating the beginning of differentiation toward mesenchymal and neuronal fates. By 35ss, migration is restricted to caudal levels, and fully differentiated chromaffin cells are observed. Conclusions: We find that some markers show patterns that differ from other tetrapods. For example, the antibody HNK-1 labels three NCC streams from the hindbrain while some comparable reptile studies describe four. However, the information emerging from all markers combined shows that the overall spatiotemporal distribution of NCCs in the common wall lizard is largely conserved with that of other tetrapods. Our study highlights the dynamic nature of seemingly canonical marker genes and provides the first description of spatiotemporal NCC dynamics in a lacertid lizard.
(Less)
- author
- Pranter, Robin
LU
and Feiner, Nathalie
LU
- organization
- publishing date
- 2024
- type
- Contribution to journal
- publication status
- epub
- subject
- keywords
- cell migratory routes, gene expression patterns, HNK-1, squamate reptiles
- in
- Developmental Dynamics
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85209823204
- pmid:39560189
- ISSN
- 1058-8388
- DOI
- 10.1002/dvdy.758
- language
- English
- LU publication?
- yes
- id
- fe768a25-a96c-406a-872f-b812273da739
- date added to LUP
- 2025-02-04 10:34:09
- date last changed
- 2025-07-08 23:13:40
@article{fe768a25-a96c-406a-872f-b812273da739,
abstract = {{<p>Background: Neural crest cells (NCCs) are migratory embryonic stem cells that give rise to a diverse set of cell types. Here we describe the dynamic distribution of NCCs in developing embryos of the common wall lizard Podarcis muralis inferred from 10 markers. Our aim is to provide insights into the NCC development of lacertid lizards and to infer evolutionary modifications by comparisons to other tetrapods. Results: NCC migration is ongoing at oviposition, following three streams in the head and multiple in the trunk. From 21ss, we observe expression patterns indicating the beginning of differentiation toward mesenchymal and neuronal fates. By 35ss, migration is restricted to caudal levels, and fully differentiated chromaffin cells are observed. Conclusions: We find that some markers show patterns that differ from other tetrapods. For example, the antibody HNK-1 labels three NCC streams from the hindbrain while some comparable reptile studies describe four. However, the information emerging from all markers combined shows that the overall spatiotemporal distribution of NCCs in the common wall lizard is largely conserved with that of other tetrapods. Our study highlights the dynamic nature of seemingly canonical marker genes and provides the first description of spatiotemporal NCC dynamics in a lacertid lizard.</p>}},
author = {{Pranter, Robin and Feiner, Nathalie}},
issn = {{1058-8388}},
keywords = {{cell migratory routes; gene expression patterns; HNK-1; squamate reptiles}},
language = {{eng}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Developmental Dynamics}},
title = {{Spatiotemporal distribution of neural crest cells in the common wall lizard Podarcis muralis}},
url = {{http://dx.doi.org/10.1002/dvdy.758}},
doi = {{10.1002/dvdy.758}},
year = {{2024}},
}