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Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis

Lekberg, Ylva; Rosendahl, Soren; Michelsen, Anders and Olsson, Pål Axel LU (2013) In Plant and Soil 368(1-2). p.547-555
Abstract
Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage... (More)
Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts. Conclusions Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arbuscular mycorrhiza, Carbon allocation, Grassland, Lipid analysis, Season, Stable isotope probing
in
Plant and Soil
volume
368
issue
1-2
pages
547 - 555
publisher
Springer
external identifiers
  • wos:000321641700041
  • scopus:84879170765
ISSN
0032-079X
DOI
10.1007/s11104-012-1534-7
project
BECC
language
English
LU publication?
yes
id
01751022-e616-493a-a3a8-904bc26bf901 (old id 3974096)
date added to LUP
2013-08-21 16:26:53
date last changed
2019-10-13 03:48:23
@article{01751022-e616-493a-a3a8-904bc26bf901,
  abstract     = {Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts. Conclusions Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed.},
  author       = {Lekberg, Ylva and Rosendahl, Soren and Michelsen, Anders and Olsson, Pål Axel},
  issn         = {0032-079X},
  keyword      = {Arbuscular mycorrhiza,Carbon allocation,Grassland,Lipid analysis,Season,Stable isotope probing},
  language     = {eng},
  number       = {1-2},
  pages        = {547--555},
  publisher    = {Springer},
  series       = {Plant and Soil},
  title        = {Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis},
  url          = {http://dx.doi.org/10.1007/s11104-012-1534-7},
  volume       = {368},
  year         = {2013},
}