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IDH1 and IDH2 mutations in pediatric acute leukemia

Andersson, A K LU orcid ; Miller, D W ; Lynch, J A ; Lemoff, A S ; Cai, Z ; Pounds, S B ; Radtke, I ; Yan, B ; Schuetz, J D and Rubnitz, J E , et al. (2011) In Leukemia 25(10). p.1570-1577
Abstract

To investigate the frequency of isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) mutations in pediatric acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL), we sequenced these genes in diagnostic samples from 515 patients (227 AMLs and 288 ALLs). Somatic IDH1/IDH2 mutations were rare in ALL (N=1), but were more common in AML, occurring in 3.5% (IDH1 N=3 and IDH2 N=5), with the frequency higher in AMLs with a normal karyotype (9.8%). The identified IDH1 mutations occurred in codon 132 resulting in replacement of arginine with either cysteine (N=3) or histidine (N=1). By contrast, mutations in IDH2 did not affect the homologous residue but instead altered codon 140, resulting in replacement of arginine with either glutamine (N=4)... (More)

To investigate the frequency of isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) mutations in pediatric acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL), we sequenced these genes in diagnostic samples from 515 patients (227 AMLs and 288 ALLs). Somatic IDH1/IDH2 mutations were rare in ALL (N=1), but were more common in AML, occurring in 3.5% (IDH1 N=3 and IDH2 N=5), with the frequency higher in AMLs with a normal karyotype (9.8%). The identified IDH1 mutations occurred in codon 132 resulting in replacement of arginine with either cysteine (N=3) or histidine (N=1). By contrast, mutations in IDH2 did not affect the homologous residue but instead altered codon 140, resulting in replacement of arginine with either glutamine (N=4) or tryptophan (N=1). Structural modeling of IDH2 suggested that codon 140 mutations disrupt the enzyme's ability to bind its substrate isocitrate. Accordingly, recombinant IDH2 R140Q/W were unable to carry out the decarboxylation of isocitrate to α-ketoglutarate (α-KG), but instead gained the neomorphic activity to reduce α-KG to R(-)-2-hydroxyglutarete (2-HG). Analysis of primary leukemic blasts confirmed high levels of 2-HG in AMLs with IDH1/IDH2 mutations. Interestingly, 3/5 AMLs with IDH2 mutations had FLT3-activating mutations, raising the possibility that these mutations cooperate in leukemogenesis.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Base Sequence, Child, Chromatography, Ion Exchange, DNA Primers, Humans, In Situ Hybridization, Fluorescence, Isocitrate Dehydrogenase/genetics, Leukemia, Myeloid, Acute/enzymology, Mutagenesis, Site-Directed, Mutation, Polymerase Chain Reaction, Precursor Cell Lymphoblastic Leukemia-Lymphoma/enzymology, Tandem Mass Spectrometry
in
Leukemia
volume
25
issue
10
pages
8 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:80054035931
  • pmid:21647154
ISSN
1476-5551
DOI
10.1038/leu.2011.133
language
English
LU publication?
no
id
0261d57d-9e36-4946-b69f-c4d360a8224c
date added to LUP
2019-06-19 14:10:47
date last changed
2024-09-18 02:52:56
@article{0261d57d-9e36-4946-b69f-c4d360a8224c,
  abstract     = {{<p>To investigate the frequency of isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) mutations in pediatric acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL), we sequenced these genes in diagnostic samples from 515 patients (227 AMLs and 288 ALLs). Somatic IDH1/IDH2 mutations were rare in ALL (N=1), but were more common in AML, occurring in 3.5% (IDH1 N=3 and IDH2 N=5), with the frequency higher in AMLs with a normal karyotype (9.8%). The identified IDH1 mutations occurred in codon 132 resulting in replacement of arginine with either cysteine (N=3) or histidine (N=1). By contrast, mutations in IDH2 did not affect the homologous residue but instead altered codon 140, resulting in replacement of arginine with either glutamine (N=4) or tryptophan (N=1). Structural modeling of IDH2 suggested that codon 140 mutations disrupt the enzyme's ability to bind its substrate isocitrate. Accordingly, recombinant IDH2 R140Q/W were unable to carry out the decarboxylation of isocitrate to α-ketoglutarate (α-KG), but instead gained the neomorphic activity to reduce α-KG to R(-)-2-hydroxyglutarete (2-HG). Analysis of primary leukemic blasts confirmed high levels of 2-HG in AMLs with IDH1/IDH2 mutations. Interestingly, 3/5 AMLs with IDH2 mutations had FLT3-activating mutations, raising the possibility that these mutations cooperate in leukemogenesis.</p>}},
  author       = {{Andersson, A K and Miller, D W and Lynch, J A and Lemoff, A S and Cai, Z and Pounds, S B and Radtke, I and Yan, B and Schuetz, J D and Rubnitz, J E and Ribeiro, R C and Raimondi, S C and Zhang, J and Mullighan, C G and Shurtleff, S A and Schulman, B A and Downing, J R}},
  issn         = {{1476-5551}},
  keywords     = {{Base Sequence; Child; Chromatography, Ion Exchange; DNA Primers; Humans; In Situ Hybridization, Fluorescence; Isocitrate Dehydrogenase/genetics; Leukemia, Myeloid, Acute/enzymology; Mutagenesis, Site-Directed; Mutation; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma/enzymology; Tandem Mass Spectrometry}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1570--1577}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Leukemia}},
  title        = {{IDH1 and IDH2 mutations in pediatric acute leukemia}},
  url          = {{http://dx.doi.org/10.1038/leu.2011.133}},
  doi          = {{10.1038/leu.2011.133}},
  volume       = {{25}},
  year         = {{2011}},
}