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In vitro imaging of b-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

Miceli, Vitale; Meli, Valeria; Blanchard-Desce, Mireille; Bsaibess, Talia; Pampalone, Mariangela; Conaldi, Pier Giulio; Caltagirone, Claudia; Obiols-Rabasa, Marc LU ; Schmidt, Judith and Talmon, Yeshayahu, et al. (2016) In RSC Advances 6. p.62119-62127
Abstract
To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed... (More)
To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging. (Less)
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RSC Advances
volume
6
pages
9 pages
publisher
Royal Society of Chemistry
external identifiers
  • Scopus:84977274180
ISSN
2046-2069
DOI
10.1039/C6RA09616F
language
English
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yes
id
02a02fbf-1668-4693-9e16-9985dd0cbbbb
date added to LUP
2016-07-20 15:39:18
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2017-02-26 04:40:06
@article{02a02fbf-1668-4693-9e16-9985dd0cbbbb,
  abstract     = {To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging.},
  author       = {Miceli, Vitale and Meli, Valeria and Blanchard-Desce, Mireille and Bsaibess, Talia and Pampalone, Mariangela and Conaldi, Pier Giulio and Caltagirone, Claudia and Obiols-Rabasa, Marc and Schmidt, Judith and Talmon, Yeshayahu and Casu, Anna and Murgia, Sergio},
  issn         = {2046-2069},
  language     = {eng},
  month        = {06},
  pages        = {62119--62127},
  publisher    = {Royal Society of Chemistry},
  series       = {RSC Advances},
  title        = {In vitro imaging of b-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles},
  url          = {http://dx.doi.org/10.1039/C6RA09616F },
  volume       = {6},
  year         = {2016},
}